Endolysin is a generic term describing an enzyme that degrades the bacterial peptidoglycan cell wall, resulting in lysis of the bacterial cell. ref Young, R. 1992 Bacteriophage lysis mechanism and regulation. Microbiol. Rev. 56 430 81. ref At the end of the bacteriophage replication cycle, bacteriophages have to release their newly formed Progeny genetic descendant progeny . The major barrier to overcome is the peptidoglycan . To degrade this layer, bacteriophages contain endolysins. Depending on the bond these enzymes cleave, we can distinguish muramidase s lysozyme , transglycosylase s, amidase s or endopeptidase s. References reflist 2 Category Bacteriophages Enzyme stub ... more details
orphan date May 2011 The Phage ligand technology is a technology to detect, bind and remove bacteria and bacterial toxin s by using highly specific bacteriophage derived proteins. ref http www.hyglos.de en technology technological background.html Technological background ref Origins The Bacteriophages Attachment and penetration host recognition of bacteriophages occur via bacteria binding proteins that have strong binding affinities to specific protein or carbohydrate structures on the surface of the bacterial host. At the end of the Bacteriophages Replication infection life cycle the bacteria lysing Endolysin is synthesized and degrades the bacterial peptidoglycan cell wall , resulting in lysis and therefore killing of the bacterial cell. Applications Bacteriophage derived proteins are used for detection and removal of bacteria ref Kretzer JW, Lehmann R, Banz M, Kim KP, Korn C. Loessner MJ 2007 Use of high affinity cell wall binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. Appl Environ Microbiol 73 1992 2000 ref ref Rozand, C., Feng, P. C. H. 2009 . Specifity analysis of a novel phage derived ligand in an Enzyme linked fluorescent assay for detection of Escherichia coli O157 H7. J. food protection 72, 1078 1081. ref and bacterial components especially Endotoxins Endotoxin contamination endotoxin contaminations in food, ref citation title Bacteriophages New Applications in Food Microbiology journal bioFood n 3 December 2006, page 2 url http www.biomerieux usa.com upload bioFood 20Dec 2006 1.pdf ref ref http www.hyglos.de en technology applications.html Applications of the phage ligand technology ref human diagnostics and decolonization of bacteria causing nosocomial infections e.g. MRSA . Protein modifications allow the biotechnological adaption to specific requirements. See also Affinity magnetic separation References references DEFAULTSORT Phage Ligand Technology Category Laboratory techniques Category Molecular bio ... more details
and Escherichia coli endolysin . ref name PUB00003216 cite journal author Matthews BW, Weaver LH title ... heteropolymers of prokaryotic cell wall s. E. coli endolysin also functions in bacterial cell ... more details
determined by the sequence of the protein, suddenly makes holes in the membrane. R is an endolysin ... proteins that form a complex that somehow destroys the outer membrane, after the endolysin has ... more details
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