Image Hunchback in situ.jpg thumb In situhybridization of wild type Drosophila embryos at different developmental stages for the RNA from a gene called hunchback. In situhybridization ISH is a type of Hybridisation molecular biology hybridization that uses a labeled complementary DNA or RNA strand i.e., Hybridization probe probe to localize a specific DNA or RNA sequence in a portion or section of tissue biology tissue in situ , or, if the tissue is small enough e.g. plant seeds, Drosophila embryos , in the entire tissue whole mount ISH . This is distinct from immunohistochemistry , which usually localizes proteins in tissue sections. DNA ISH can be used to determine the gene mapping structure of chromosomes. Fluorescent in situhybridizationFluorescent DNA ISH FISH can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH hybridization histochemistry is used ... cite journal pmid 9021518 year 1997 last1 Jin first1 L last2 Lloyd first2 RV title In situhybridization ... snge Protocols ISHH ISHH.html Comprehensive and annotated in situhybridization histochemistry External links MeshName In SituHybridization http www.cshprotocols.org cgi content full 2008 3 pdb.prot4944 Whole Mount In SituHybridization of RNA Probes to Plant Tissues http www.cshprotocols.org cgi ... Different Fluorochromes http www.cshprotocols.org cgi content full 2006 21 pdb.prot4519 Transcript In SituHybridization of Whole Mount Embryos for Phenotype Analysis of RNAi Treated Drosophila in situ ... For hybridization histochemistry , sample cells and tissues are usually treated to fix the target ... radio , fluorescent or antigen labeled bases e.g., digoxigenin is localized and quantified ... Category Laboratory techniques Category Biological techniques and tools ar de In situ Hybridisierung es Hibridaci n in situ fr Hybridation in situ he ja In situ sv In situ hybridisering ... more details
Orphan date April 2012 Context date September 2009 CISH, or chromogenic in situhybridization , is a process in which a labeled complementary DNA or RNA strand is used to localize a specific DNA or RNA sequence in a tissue specimen. CISH methodology may be used to evaluate gene amplification, Deletion genetics gene deletion , Chromosomal translocation chromosome translocation , and chromosome number. CISH uses conventional peroxidase or alkaline phosphatase reactions visualized under a standard bright field microscope, and is applicable to formalin fixed, paraffin embedded FFPE tissues, blood or bone marrow smears, metaphase chromosome spreads, and fixed cells. ref http www.invitrogen.com site us en home Products and Services Applications Clinical and Diagnostic Applications Clinical Pathology CP Misc SPOT Light HER2 CISH Kit CISH Technology Overview.html CISH Overview ref CISH is an alternative to fluorescent in situhybridization . References reflist References Reflist DEFAULTSORT Chromogenic In SituHybridization Category Molecular biology techniques ... more details
FISH probes. Preparation and Hybridization Process File FISH Fluorescent In SituHybridization .jpg ... PolyPro pathology DEFAULTSORT Fluorescent In SituHybridization Category Anatomical pathology Category ... Chromosom da ymas nl Fluorescent in situhybridization ja in situ pl Fluorescencyjna ... is present. FISH fluorescence in situ hybridisation molecular biology hybridization is a cytogenetics ... or absence of specific DNA DNA sequence sequences on chromosome s. FISH uses hybridization probe fluorescent ... large as to impede the hybridization process. The probe is Fluorescent tag tagged directly ... in situhybridization studies in infertile patients indications, study approach, and clinical relevance ... Towards a cellular multi parameter analysis platform fluorescence in situhybridization FISH on microhole ... Commons category In situhybridization Molecular cytogenetics Virtual Karyotype Happy mapping Gallery ..., Rudolf Amann 2002 Fluorescence In SituHybridization and Catalyzed Reporter Deposition for the Identification ... in SituHybridization Information on http www.olympusfluoview.com applications fiberfish.html ... http www.biovisible.com photopage.shtml Fluorescence in situHybridization Photos of bacteria Rational ... complementarity. Fluorescence microscopy can be used to find out where the fluorescent probe ... images. If the fluorescent signal is weak, amplification of the signal may be necessary in order to exceed the detection threshold of the microscope . Fluorescent signal strength depends on many ... within cells and tissues rely on the use of a reporter gene , such as one expressing green fluorescent ... of differently colored probes. A range of colors in mixtures of fluorescent dyes can be detected, so ... fluorescent dye colors, ratios of probe mixtures can be used to create secondary colors. Similar to comparative genomic hybridization , the probe mixture for the secondary colors is created by mixing ... imaged through the application of multiple short singly labeled hybridization probe oligonucleotide ... more details
Wiktionary situ Wikisearchbox situ text2 situs The words situ and situs are Latin for site or location . They also have other meanings Situ means lake in Sundanese language Sundanese . Situ can refer to in situ , Latin phrase meaning on site or in place In situ compaction , a term for spray forming or spray casting In situ oxidation , in situ chemical oxidation ISCO is a technique to clean up some types of environmental contamination In situ polymerization , in polymer chemistry, means in the polymerization mixture Ductal carcinoma in situ , a form of cancer Lobular carcinoma in situ LCIS , a condition caused by unusual cells in the lobules of the breast rarely cancer Situ Babakan Lake, where water flows in from the Ciliwung River Situ Gintung , an artificial lake near to the town of Cirendeu People with the Chinese compound surname Situ Szeto Wah Situ Hua , Pinyin name of Szeto Wah, a chairman of The Hong Kong Alliance Situ Panchen , the 8th Tai Situ Rinpoche, an influential Tibetan De Situ Albanie , first of seven Scottish documents found in Poppleton Manuscript De Situ Britanniae , a fictional description of the peoples and places of ancient Britain Melanoma in situ , early stage where most melanomas are detected, before they become elevated Venus In Situ Explorer VISE , a mission to Venus proposed by NASA Tai Situ , or Tai Situpa, name of Chokyi Gyaltsen as first incarnation to bear the title Tai Situ Shakoor v Situ , a court case List of colossal sculpture in situ , roster of large sculptures created in place Lasik , laser assisted in situ keratomileusis , a type of refractive surgery for correcting myopia, hyperopia, etc. Situs can refer to Situs law , a legal term meaning site where a law applies Lex situs , refers to the law of the place in which property is situated Situs solitus , refers to the normal position of thoracic and abdominal organs Dextrocardia Situs Inversus , medical term for heart being a mirror image situated on the right side. Analysis Situs ... more details
sources February 2012 italic title wiktionary In situ IPAc en n s tj u is a Latin phrase ... must be tested in situ , or in place, to confirm everything functions properly as a system. Individually .... Special test equipment is available for this in situ testing. Archaeology This section is linked ... , in situ refers to an artifact that has not been moved from its original place of deposition. In other words, it is stationary, meaning still. An artifact being in situ is critical to the interpretation ... and display. An artifact that is not discovered in situ is considered out of context and as not providing ... scientists with an example of types and locations of in situ artifacts yet to be discovered. The label In situ indicates only that the object has not been newly moved. Thus, an archaeological in situ ... war, a traded item, or otherwise of foreign origin. Consequently, the in situ find site may still ... uniform or horizontal, or in land cleared or tilled for farming. The term In situ is often ... in ancient times. Art In art, in situ refers to a work of art made specifically for a host site, or that a work ... in Milky Way our Galaxy , as well as those in other massive galaxies, might have formed in situ ... thumb Live individual of the sea snail Natica hebraea photographed in situ In biology , in situ ... in situ when used in laboratory science such as cell science can mean something intermediate ... anatomy organ intact and under perfusion may be in situ investigation. This would not be in vivo as the donor .... To bridge the dichotomy of benefits associated with both methodologies, in situ experimentation ... of in vivo experimentation. In conservation of Germplasm genetic resources , in situ conservation in situ conservation also on site Conservation movement conservation is the process of protecting ... to Ex situ conservation ex situ conservation also off site conservation . Chemistry and chemical engineering In chemistry, in situ typically means in the reaction mixture. There are numerous ... more details
, such as bacteria , archaea , and eukaryotes via fluorescence in situhybridization . ref cite journal ...Refimprove date December 2009 In molecular biology , a hybridization probe is a fragment of DNA or RNA of variable length usually 100 1000 bases long , which is used in DNA or RNA samples to detect the presence of nucleotide sequences the DNA target that are Complementarity molecular biology complementary to the sequence in the probe. The probe thereby hybridizes to single stranded nucleic acid DNA or RNA whose base sequence allows probe target base pair ing due to complementarity between the probe and target. The labeled probe is first denaturation biochemistry denatured by heating or under alkaline conditions such as exposure to sodium hydroxide into single stranded DNA ssDNA and then hybridized to the target ssDNA Southern blot ting or RNA northern blot ting immobilized on a membrane or fluorescent in situhybridization in situ . To detect Nucleic acid hybridizationhybridization of the probe to its target sequence, the probe is tagged or labelled with a molecular marker of either radioactive or more recently fluorescent molecules commonly used markers are Isotopes of phosphorus sup ... or high similarity depends on how stringent the hybridization conditions were applied high stringency, such as high hybridization temperature and low salt in hybridization buffers, permits only hybridization ... temperature and high salt, allows hybridization when the sequences are less similar. Hybridization ..., W. and Schleifer, K. H. year 1995 title Phylogenetic identification and in situ detection of individual ... in situ of Nevskia ramosa url journal Appl. Environ. Microbiol. volume 64 pages 1895 1901 ... updated, thus the possibility of a hybridization event between a specifically designed probe based ... science, hybridization probes are used, for example, for detection of short tandem repeats ... of DNA profiling analysis. References references DEFAULTSORT Hybridization Probe Category Molecular ... more details
Sequencing by Hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA . Typically used for looking for small changes relative to a known DNA sequence . The binding of one strand of DNA to its complementary strand in the DNA double helix aka hybridization is sensitive to even single base mismatches when the hybrid region is short or if specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notable via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single base variations. The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation as well as pore based methods . However hybridization of oligonuceleotides is still used in some sequencing schemes, including hybridization assisted pore based sequencing, and reversible hybridization. Examples of commercial systems Affymetrix true sequencing by hybridization http www.nabsys.com NABsys Hybridization assisted pore based sequencing http www.completegenomics.com Complete Genomics Inc. reversible hybridization of probes that call out a single base with each hybridization References Preparata FP, Upfal E. Sequencing by hybridization at the information theory bound an optimal algorithm. J Comput Biol. 2000 7 3 4 621 30. PMID 11108482 Drmanac R et al. Sequencing by hybridization SBH advantages, achievements, and opportunities. Adv Biochem Eng Biotechnol. 2002 77 75 101. PMID 12227738 Hanna GJ et al. Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase . J Clin Microbiol. 2000 July 38 7 2715 2721. PMID 10878069 Church, George M. Genomes for all. Scientific American. 2006 Jan 294 1 52. DOI 10.1038 scientificamerican0106 46 PMID 16468433 See also ... more details
Unreferenced date December 2009 In molecular biology and biotechnology , a fluorescent tag is a part of a molecule that researchers have attached chemically to aid in detection of the molecule to which it has been attached. The tag is some kind of fluorescent molecule also known as fluorophore . Ethidium bromide , Fluorescein and Green fluorescent protein are common tags. See also Molecular tagging velocimetry Spectrophotometer for Nucleic Acid Measurements DEFAULTSORT Fluorescent Tag Category Molecular biology Biochem stub ... more details
Refimprove article date June 2010 Fluorescent labelling is the process of covalently attaching a fluorophore to another molecule, such as a protein or nucleic acid . This is generally accomplished using a reactive derivative of the fluorophore that selectively binds to a functional group contained in the target molecule. The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes for detection of a particular target. ref http pharmaxchange.info press 2011 01 fluorescent labeling of biomolecules with organic probes Presentation on Fluorescent labelling of biomolecules with organic probes PharmaXChange.info ref Detection Fluorescent labels are generally used for detection of a protein or other labeled molecule via a fluorescence microscope , flow cytometer or some other fluorescence reading instrument. These can be useful in localization of a target within a cell, flow cytometry FACS analysis, western blot assays, and other immunoanalytical methods. Labelling techniques Fluorescent labelling is accomplished using a chemically reactive derivative of a fluorophore. Common reactive groups include Isothiocyanate derivatives such as Fluorescein isothiocyanate FITC and TRITC derivatives of fluorescein and rhodamine are reactive towards primary amines to form a thioureido linkage between the compound of interest and the dye. Succinimidyl esters such as NHS fluorescein are reactive towards amino groups to form an amido bond. Maleimide activated fluorophores such as fluorescein 5 maleimide readily react with sulfhydryl ... between a fluorophore and a labelled molecule. Following a fluorescent labelling reaction, it is often ... for the hydrophobic properties of fluorescent dyes are sometimes used. Reactive fluorescent dyes are available ... kits that contain all the components to carry out a labelling reaction. Common fluorescent dyes ... DEFAULTSORT Fluorescent Labelling Category Molecular biology uk ... more details
Infobox single Name Fluorescent Adolescent Cover Fluorescent Adolescent.JPG Artist Arctic Monkeys from Album Favourite Worst Nightmare B side The Bakery br Plastic Tramp br Too Much to Ask Released 4 July 2007 Japan br 9 July 2007 UK Format Gramophone record 7 , 10 , Compact Disc single CD Recorded December 2006 at Miloco Studios , London Genre Indie rock , ref cite web url http www.bbc.co.uk music ... Ford, Mike Crossey Last single Brianstorm br 2007 This single Fluorescent Adolescent br 2007 Next ... Brianstorm Teddy Picker D Is for Dangerous Balaclava Fluorescent Adolescent Only Ones Who Know ... Fluorescent Adolescent is a song by the English indie rock band Arctic Monkeys , released as the second ..., discarded all the naughty nights for niceness, landed in a very common crisis . Fluorescent Adolescent ... Arctic Monkeys http www.arctic monkeys.com fluorescent adolescent.php Arctic Monkeys ref The music ... is also printed inside the CD release of Fluorescent Adolescent. The lines such as out of control ... yes title1 Fluorescent Adolescent length1 3 03 lyrics1 Alex Turner musician Alex Turner , Johanna ... 7 lyrics credits yes music credits yes title1 Fluorescent Adolescent length1 3 03 lyrics1 Alex Turner ... align center 5 On downloads alone, Fluorescent Adolescent charted in the UK Singles Chart at 55 ... for the week commencing 16 July 2007. Since 27 June 2007, Fluorescent Adolescent has received significant ... links http www.arctic monkeys.com fluorescent adolescent.php Fluorescent Adolescent music video at ArcticMonkeys.com download link http www.youtube.com watch?v ma9I9VBKPiw Fluorescent Adolescent ... uk singles 10 12 07 fluorescent adolescent Fluorescent Adolescent mini site Arctic Monkeys singles Category 2007 singles Category Arctic Monkeys songs Category Songs written by Alex Turner es Fluorescent Adolescent it Fluorescent Adolescent nah Fluorescent Adolescent nl Fluorescent Adolescent pl Fluorescent Adolescent pt Fluorescent Adolescent ru Fluorescent Adolescent ... more details
Situ Panchen full name Situ Panchen Ch gyi Jungney 1700 1774 , also known as the 8th Tai Situ Rinpoche , was an influential Tibet an painter, writer and medical innovator as well as a notable figure in the histories of Karma Kagyu and the Kingdom of D g , where he served as senior court chaplain. ref name Rubin cite web url http www.rmanyc.org programs events situ panchen.xml?context programs events situ panchen.xml title Situ Panchen Creation and Cultural Engagement in 18th Century Tibet publisher Rubin Museum of Art accessdate 2009 02 15 Dead link date October 2010 bot H3llBot ref A student of Katok Tsewang Norbu , Situ Panchen is known for his role in the non sectarian Rime movement of Tibetan Buddhism , as he was instrumental in helping in the growing acceptance of Zhentong according to 2003 s The Budda from Dolpo , i n the end it would be Situ more than anyone who would create the environment for the widespread acceptance of the Zhentong theories in the next century. ref cite book title The Buddha from Dolpo A Study of the Life and Thought of the Tibetan Master Dolpopa Sherab Gyaltsen first Cyrus last Stearns publisher Motilal Banarsidass Publ., year 2003 isbn 8120818334 page 76 url http books.google.com books?id zYiWkLTrU3oC&pg PA75&dq Situ Panchen&client firefox a PPA76,M1 ref As a painter, Situ Panchen acknowledged the influence of Chinese artists, utilizing not only color and form common to their work but also setting. ref cite book title Empire of Emptiness Buddhist ... PA146&dq Situ Panchen&client firefox a PPA145,M1 ref An exhibition of Situ Panchen s work, accompanied ... day conference included focus on such topics as Situ Panchen s influence on monastic art as far ... . NAME Situ Panchen ALTERNATIVE NAMES SHORT DESCRIPTION DATE OF BIRTH 1700 PLACE OF BIRTH DATE OF DEATH 1774 PLACE OF DEATH DEFAULTSORT Situ Panchen Category 1700 births Category 1774 deaths Category ... Tai Situ tulkus Category 18th century Tibetan people de Ch kyi Chungne nl Ch kyi Jungne ... more details
Multiple issues cleanup April 2009 refimprove April 2009 orphan January 2011 In polymer chemistry, in situ means in the polymerization mixture. There are numerous unstable oligomer s molecule s which must be synthesized in situ i.e. in the reaction mixture but cannot be isolated on their own for use in various processes. Example of this method include urea formaldehyde UF and melamine formaldehyde MF encapsulation systems.In such type of in situ polymerization a chemical encapsulation technique is involved very similar to interfacial coating. The distinguishing characteristic of in situ polymerization is that no reactants are included in the core material. All polymerization occurs in the continuous phase, rather than on both sides of the interface between the continuous phase and the core material. Category Polymers Polymer stub ... more details
File Fluorescent lamps artistic.jpg thumb Fluorescent lamps File Leuchtstofflampen chtaube050409.jpg thumb Top, two compact fluorescent lamp s. Bottom, two linear fluorescent lamps. A matchstick, left ... required on all fluorescent bulbs that contain mercury. ref http www.ecy.wa.gov mercury mercury light ... 4637 3, page 21 12 ref A fluorescent lamp or fluorescent tube is a gas discharge lamp that uses electricity ... light . A fluorescent lamp converts electrical power into useful light more efficiently than ... to regulate the Electric current current through the lamp. While larger fluorescent lamps have been mostly used in commercial or institutional buildings, the compact fluorescent lamp is now available ... States Environmental Protection Agency classifies fluorescent lamps as hazardous waste, and recommends ... Edison briefly pursued fluorescent lighting for its commercial potential. He invented a fluorescent ... , but although it received a patent in 1907, ref patent US 865367 Fluorescent Electric Lamp ref it was not put ... in the 1890s, devising high frequency powered fluorescent bulbs that gave a bright greenish light, but as with Edison s devices, no commercial success was achieved. Although Edison lost interest in fluorescent ... fluorescent lamps, they were considerably more complicated than an incandescent bulb. ref ... to fluorescent coatings in the 1920s. Pages 388 391 discuss the development of the commercial fluorescent at General Electric in the 1930s. ref Its success contributed to General Electric s motivation ... were to be of considerable value when the firm took up fluorescent lighting more than two decades ... a means of illumination that also can be seen as a precursor to the modern fluorescent lamp ... 1930s they received limited use for large scale illumination. Some of them employed fluorescent ... vapor lamps also anticipated the fluorescent lamp in their incorporation of a ballast to maintain ... lighting was relevant to the development of fluorescent lighting, however, as Claude s improved electrode ... more details
for the story that ran in Heavy Metal magazine Heavy Metal Fluorescent Black comics Infobox album See Wikipedia WikiProject Albums Name Fluorescent Black Type studio Artist Antipop Consortium Cover Flourescent Black.jpg Alt Released September 28, 2009 Recorded 2008 Genre Hip hop Length Label Big Dada Producer Earl Blaize , High Priest , M.Sayyid Last album Antipop Consortium vs. Matthew Shipp br 2003 This album Fluorescent Black br 2009 Next album Album ratings rev1 Allmusic rev1score rating 4.5 5 ref cite web url Allmusic class album id r1624867 pure url yes work Allmusic author Bush, John accessdate October 14, 2009 title allmusic Fluorescent Black Overview ref rev2 Drowned in Sound rev2score 8 10 ref cite web url http drownedinsound.com releases 14680 reviews 4137989 work Drowned in Sound author Gibb, Rory title Review Anti Pop Consortium Fluorescent Black Releases Releases Drowned in Sound date September 30, 2009 accessdate October 14, 2009 ref rev3 Pitchfork Media rev3score 7.0 10 ref cite web url http pitchfork.com reviews albums 13559 fluorescent black work Pitchfork Media title Pitchfork Album Reviews Anti Pop Consortium Fluorescent Black accessdate November 16, 2009 date October 8, 2009 author Harvell, Jess ref rev4 PopMatters rev4score 8 10 ref name popmatters cite web url http www.popmatters.com pm review 110151 anti pop consortium fluorescent black title Anti Pop Consortium Fluorescent Black Reviews PopMatters work PopMatters author Ross, Daniel accessdate October 14, 2009 date October 13, 2009 ref rev5 Spin magazine Spin rev5score rating 4 5 ref cite web url http www.spin.com reviews anti pop consortium fluorescent black big dada work Spin title Anti Pop Consortium, Fluorescent Black Big Dada Spin Magazine Online author Reeves, Mosi date October 23, 2009 accessdate November 16, 2009 ref Automatically generated by DASHBot Fluorescent Black is the fourth album ..., Anti Pop Consortium, Austin length16 1 28 title17 Fluorescent Black writer17 Austin, Blaize, Anti ... more details
In situ conservation is on site Conservation movement conservation or the conservation of genetic resources in natural populations of plant or animal species , such as forest genetic resources in natural populations of tree species. It is the process of protecting an endangered species endangered plant or animal species in its natural habitat ecology habitat , either by protecting or cleaning up the habitat itself, or by defending the species from predator s. It is applied to conservation of agricultural biodiversity in agroecosystem s by farmers, especially those using unconventional farming practices. Benefits One benefit of in situ conservation is that it maintains recovering populations in the surrounding where they have developed their distinctive properties. Another is that this strategy helps ensure the ongoing processes of evolution and adaptation within their environments. As a last resort, ex situ conservation may be used on some or all of the population, when in situ conservation is too difficult, or impossible. Reserves Wildlife and livestock conservation is mostly based on in situ ... other catastrophes. Agriculture In agriculture , in situ conservation techniques are an effective ... 2004. Estrategias para la conservaci n in situ de razas de ma z boliviano . SINALERC, Mar del ... articles.asp?lg 0&a cbd 08 In Situ Conservation, The Convention on Biological Diversity http www.biodiv.org convention articles.asp?lg 0&a cbd 09 Ex Situ Conservation, The Convention on Biological ... Diversity http www.bioversityinternational.org Themes Conservation and Use index.asp In situ conservation In situ conservation http www.panda.org The World Wide Fund for Nature http www.awdconservancy.org African Wild Dog Conservancy Zoos DEFAULTSORT In Situ Conservation Category Conservation ... Category Habitat ecology terminology et In situ looduskaitse es Conservaci n in situ eo Ena konservado fr Conservation in situ pl Ochrona in situ ru in situ ... more details
Infobox disease Name Carcinoma in situ Image In situ carcinoma.jpg Caption DiseasesDB ICD10 ICD9 ICDO ICDO 8010 2 OMIM MedlinePlus eMedicineSubj eMedicineTopic MeshID Carcinoma in situ CIS is an early form of cancer that is defined by the absence of invasion of tumor cells into the surrounding tissue, usually before penetration through the basement membrane . In other words, the neoplasm neoplastic cells proliferate in their normal habitat, hence the name in situ Latin for in its place . For example, carcinoma in situ of the skin, also called Bowen s disease , is the accumulation of neoplastic epidermal cells within the epidermis only, that has failed to penetrate into the deeper dermis. For this reason, CIS will usually not form a tumor . Rather, the lesion is flat in the skin, cervix, etc. or follows the existing architecture of the organ in the breast, lung, etc. . Some CIS, however, do ... more properly called Mammary ductal carcinoma ductal carcinoma in situ . Many forms of invasive ... tumor. In the TNM staging system TNM classification, carcinoma in situ is reported as T is N0M0 Cancer staging Stage 0 . Dysplasia vs carcinoma in situ vs invasive carcinoma These terms are related ... is low. Carcinoma in situ is synonymous with high grade dysplasia in most organs. The risk of transforming ... with the pap smear . Mammary ductal carcinoma Ductal carcinoma in situ DCIS of the breast ... untreated. Bowen s disease is squamous carcinoma in situ of the skin. Polyp medicine Colon polyps .... Image Ca in situ, cervix 2.jpg thumb High grade dysplasia carcinoma in situ in the uterine ... can progress to invasive cancer squamous cell carcinoma of the cervix. Treatment Carcinoma in situ is, by definition ... Tumors Category Histopathology Category Carcinoma ar de Carcinoma in situ es Carcinoma in situ fa it Carcinoma in situ nl Carcinoma in situ pl Rak w miejscu pt Carcinoma in situ ru in situ zh ... more details
Fluorescent microthermography FMT is a microscopy technique for infrared imaging of temperature distribution in small scale the achievable spatial resolution is half micrometer and temperature resolution of 0.005 kelvin K . Time dependent measurements are possible, as the fluorescence lifetime is only about 200 microseconds. A thin film of a phosphor , europium thenoyl trifluoroacetonate , is applied on the surface e.g. an integrated circuit die integrated circuit die and illuminated by ultraviolet light at 340 380 nm, stimulating fluorescence at mainly 612 nm line. The quantum efficiency of fluorescence decreases exponentially with temperature, differences in emitted light intensity can be therefore used to assess differences on surface temperature, with hot areas showing as darker. ref http books.google.com books?id OX2cHKJWCKgC&pg PA713&dq semiconductor failure microphotograph&lr &as drrb is q&as minm is 0&as miny is &as maxm is 0&as maxy is &num 50&as brr 3&cd 3 v onepage&q &f false ref References reflist tech stub Category Infrared imaging Category Microscopy Category Thermometers Category Measurement ... more details
Infobox football biography name Darnel Situ image fullname Darnel Situ Buyente height convert 1.87 m ... 2011 UTC pcupdate 13 October 2010 Darnel Situ Buyente born 18 March 1992 in Rouen is a France French ... and has Captain association football captained at all levels of youth he has played at. Situ primarily ... and is known for his robust physique and physical strength. Football career Early career Situ began ..., Situ established himself as one of the club s top youth prospects, alongside fellow defender William ... stage without a single point. While playing with the national under 16 team, Situ, alongside Stade ... ref cite news title Man Utd, Liverpool battle for Lens kid Situ Buyence url http www.tribalfootball.com content man utd liverpool battle lens kid situ buyence work Trial Football date 23 August 2008 accessdate 9 January 2010 ref Situ, at one point, met with Manchester United Coach sports manager Alex Ferguson , but a deal could not be reached. ref cite news title Interview exclusive de Darnel Situ du RC Lens url http www.espoirsdufootball.com 643 interview exclusive de darnel situ du rc lens.html ... joined Manchester City F.C. Manchester City . Lens Despite constant rumors of the Situ rejecting .... Situ made his debut in the reserves opening match against Vesoul Haute Sa ne , appearing as a substitute ... with the senior team at the start of the 2010 portion of the season, Situ made his professional debut ... in the team s 1 0 victory. Situ had a trial at Newcastle United F.C. Newcastle United and scored a goal in his debut in a reserves match 5 0 win over Gateshead Reserves. Swansea Situ agreed a two ... Swans snap up Situ from Lens url http www.swanseacity.net page Latest 0,,10354 2438127,00.html ref Several weeks later, FIFA announced that international clearance wouldn t be granted for Situ. ref cite ... infos 2012 01 10 situ index.php ancreContenu work RC Lens language French ref International career Situ is known for his impeccable leadership. He has captained several of his youth sides, which include ... more details
File Ralsko uran.JPG thumb Remains of uranium in situ leaching in Str pod Ralskem, Czech Republic In situ leaching ISL , also called in situ recovery ISR or solution mining , is a mining process used to recover minerals such as copper and uranium through boreholes drilled into a deposit, in situ . The process initially involves drilling of holes into the ore deposit. Explosive or hydraulic fracturing may be used to create open pathways in the deposit for solution to penetrate. Leaching solution is pumped into the deposit where it makes contact with the ore. The solution bearing the dissolved ore content is then pumped to the surface and processed. This process allows the extraction of metals ... cut or sub surface mining underground mining . Process In situ leach mining involves pumping of a leachate ... the solution. For uranium ores, the leachate may be acid or sodium bicarbonate . Soluble salts In situ ... Mining of Nahcolite ref In situ leaching is often used when the deposits are too deep, or the beds ... solutions. ref name sea us http www.sea us.org.au pdfs tmw00 TMW00 Oz USA.pdf Acid In Situ ... dupr.html EIA Domestic Uranium Report. ref in situ leaching uranium mines operating in the United ... In situ leach mines operating in the United States. EIA Domestic Uranium Production Report. ref ... uses in situ leaching. ISL mining accounted for 41 of the world s uranium production in 2010 ... of in situ uranium mines include The Beverley Uranium Mine , South Australia, is an operating ... in the United States Copper see also copper extraction In situ leaching of copper was done by the Chinese ... 530 R 94 031. ref Gold In situ leaching has not been used on a commercial scale for gold mining. A three year pilot program was undertaken in the 1970s to in situ leach gold ore at the Ajax mine in the Cripple ... section date November 2010 In situ leach techniques are often controversial, sometimes because ... In Situ Leach Category Mining techniques Category Economic geology Category Uranium Category ... more details
MedalTableTop MedalSport Track and field athletics MedalCountry RSA MedalCompetition Paralympic Games MedalGold 2000 Summer Paralympics 2000 Sydney Athletics at the 2000 Summer Paralympics Women s javelin throw F52 54 Javelin Throw F52 54 MedalGold 2004 Summer Paralympics 2004 Athens Athletics at the 2004 Summer Paralympics Women s javelin throw F54 55 Javelin Throw F54 55 MedalSilver 2000 Summer Paralympics 2000 Sydney Athletics at the 2000 Summer Paralympics Women s discus throw F51 54 Discus Throw F51 54 MedalBottom Zanele Situ is a Paralympian Track and field athlete from South Africa competing mainly in category F54 javelin events. Zanele has competed in three paralympics, always competing in the three throwing events, the shot putt, discus throw and javelin. Her first and most successful games were in 2000 Summer Paralympics 2000 where she won a gold in the F52 54 javelin and a silver in the F51 54 discus. Four years later in Athens she won her second javelin title in the F54 55 class. Unfortunately she was unable to win any medals in her third Paralympics in Beijing in 2008 Summer Paralympics 2008 . External links http www.paralympic.org release Main Sections Menu Sports Results paralympics search.html?sport all&games all&medal all&npc all&name Situ&fname Zanele&gender all profile on paralympic.org Persondata Metadata see Wikipedia Persondata . NAME Situ, Zanele ALTERNATIVE NAMES SHORT DESCRIPTION Paralympic athletes of South Africa DATE OF BIRTH PLACE OF BIRTH DATE OF DEATH PLACE OF DEATH DEFAULTSORT Situ, Zanele Category Paralympic athletes of South Africa Category Athletes track and field at the 2000 Summer Paralympics Category Athletes track and field at the 2004 Summer Paralympics Category Athletes track and field at the 2008 Summer Paralympics Category Paralympic gold medalists for South Africa Category Paralympic silver medalists for South Africa Category South African athletes Category Discus throwers Category Shot putters Category Javelin throwers Ca ... more details
Situ Gintung Sundanese language Sundanese , Lake Gintung was an Reservoir artificial lake near to the town of Cirendeu pronounced IPA su t ir nd in Tangerang Tangerang District , Indonesia . It was formed by a dam up to convert 16 m ft high which was built by Dutch East Indies Dutch colonial authorities in 1933. The dam failed on 27 March 2009, draining the lake, with resulting floods killing at least 100 people. ref name Negligence cite news url http www.thejakartaglobe.com justAdded walhi cites 6 officials for dam negligence 272142 title Walhi Cites 6 Officials For Dam Negligence work Jakarta Globe date 7 April 2009 accessdate 7 April 2009 ref History The lake and dam are located in the suburb of Cirendeu, Ciputat in Tangerang District. ref name bbc 2009 03 29 cite news url http news.bbc.co.uk 1 hi world asia pacific 7970438.stm title Indonesia crews resume dam rescue date 29 March 2009 work BBC accessdate 29 March 2009 ref The dam was originally built of earth by Dutch colonial authorities in 1933 and was up to convert 16 m ft high. ref name jakarta cite news url http www.thejakartaglobe.com justAdded yet another tragedy strikes the nation 270868 title Yet Another Tragedy Strikes the Nation date 27 March 2009 work Jakarta Globe accessdate 27 March 2009 ref It was made from earth compacted into a wall convert 16 m ft high and the reservoir held at least convert 2000000 m3 cuft of water. ref name bbc 2009 03 27 cite news url http news.bbc.co.uk 1 hi world asia pacific 7967205.stm ... image name A map of the flooded area name 2009 Situ Gintung flood duration 27 March 2009 total ... people, and 5 more were unaccounted for. ref http www.who.int hac crises idn sitreps situ gintung 30march2009 en index.html Collapsed Dam of Situ Gintung, Tangerang, Banten Province ref The waters also ... Dam disasters Category Floods in Indonesia zh min nan Situ Gintung de Situ Gintung es Situ Gintung fa ko id Situ Gintung ms Situ Gintung nl Situ Gintung th vi Situ Gintung ... more details
Subtractive hybridization is a technology that allows for Polymerase chain reaction PCR based amplification of only Complementary DNA cDNA fragments that differ between a control driver and experimental transcriptome . Differences in relative abundance of transcripts are highlighted, as are genetic differences between species. The technique relies on the removal of dsDNA formed by hybridization between a control and test sample, thus eliminating cDNAs or gDNA s of similar abundance, and retaining differentially expressed, or variable in sequence, transcripts or genomic sequences. Suppression subtractive hybridization has also been successfully used to identify strain or species specific DNA sequences in a variety of bacteria including Vibrio species. See also Representational Difference Analysis External links http www.evrogen.com technologies SSH.shtml Overview at evrogen.com Cite journal author Munir S, Singh S, Kaur K, Kapur V title Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells journal Biol Proced Online volume 6 issue pages 94 104 year 2004 pmid 15181476 doi 10.1251 bpo77 pmc 420231 http www.biologicalprocedures.com bpo arts 1 77 m77.htm link Cite journal author Diatchenko L, Lau Y, Campbell A, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E, Siebert P title Suppression subtractive hybridization a method for generating differentially regulated or tissue specific cDNA probes and libraries journal Proc Natl Acad Sci USA volume 93 issue 12 pages 6025 30 year 1996 pmid 8650213 doi 10.1073 pnas.93.12.6025 pmc 39182 Cite journal author Galbraith E, Antonopoulos D, White B title Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome the rumen as a model journal Environ Microbiol volume 6 issue 9 pages 928 37 year 2004 pmid 15305918 doi 10.1111 j.1462 2920.2004.00575.x Category Biotechnology ... more details
about the specific use in genomics the general phenomenon Nucleic acid thermodynamics Hybridization DNA DNA hybridization generally refers to a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. It is usually used to determine the genetic distance between two species . When several species are compared that way, the similarity values allow the species to be arranged in a phylogenetic tree it is therefore one possible approach to carrying out molecular systematics . Charles Sibley and Jon Ahlquist , pioneers of the technique, used DNA DNA hybridization to examine the phylogenetic relationships of avians the Sibley Ahlquist taxonomy and primates. ref http evolution.berkeley.edu evolibrary article 0 history 26 Genetic Similarities Wilson, Sarich, Sibley, and Ahlquist ref ref cite journal title The Phylogeny of the Hominoid Primates, as Indicated by DNA DNA Hybridization author C.G. Sibley and J.E. Ahlquist journal Journal of Molecular Evolution volume 20 pages 2 15 year 1984 doi 10.1007 BF02101980 pmid 6429338 issue 1 ref Critics argue that the technique is inaccurate for comparison of closely related species, as any attempt to measure differences between orthologous sequences between organisms is overwhelmed by the hybridization of paralogous sequences within an organism s genome. ref http personal.uncc.edu jmarks DNAHYB Dnahyb2.html DNA hybridization in the apes Technical issues ref DNA sequencing and computational comparisons of sequences is now generally the method for determining genetic distance, although the technique is still used in microbiology to help identify bacteria. ref cite journal title Use of checkerboard DNA DNA hybridization to study complex microbial ecosystems author S.S. Socransky, A.D. Haffajee, C. Smith, L. Martin, J.A. Haffajee, N.G. Uzel, J. M. Goodson journal Oral Microbiology and Immunology ... hybridization sample serves as a control . These results are combined to determine the degree of genetic ... more details
Array comparative genomic hybridization also CMA , Chromosomal microarray analysis , microarray based comparative genomic hybridization , array CGH , a CGH , aCGH , or virtual karyotype is a technique to detect genomic copy number variation s at a higher resolution level than chromosome based comparative genomic hybridization CGH . ref cite journal journal Drug Discov Today year 2008 title The array CGH and its clinical applications author Shinawi M, Cheung SW doi 10.1016 j.drudis.2008.06.007 pmid 18617013 volume 13 pages 760 70 issue 17 18 ref Process DNA from a test sample and normal reference sample are labelled differentially, using different fluorophores , and nucleic acid hybridization hybridized to several thousand probes. The probes are derived from most of the known genes and non coding regions of the genome , printed on a glass slide. The fluorescence intensity of the test and of the reference DNA is then measured, to calculate the ratio between them and subsequently the copy number changes for a particular location in the genome. Efficiency Using this method, copy number changes at a level of 5 10 kilobase s of DNA sequences can be detected. ref cite journal last Ren first H title BAC based PCR fragment microarray high resolution detection of chromosomal deletion and duplication breakpoints journal Human Mutation url http www.ncbi.nlm.nih.gov pubmed 15832308 ref Today even high resolution CGH HR CGH arrays are accurate to detect structural variation s SV at resolution of 200 bp. ref cite journal journal Proc. Natl. Acad. Sci. 103 4534 39 year 2006 title High resolution mapping of DNA copy alterations in human chromosome 22 using high density tiling oligonucleotide ... in the hybridization to the array element as the size of the genomes increase. This issue may ... also Comparative genomic hybridization Genome References reflist External links http www.nslij genetics.org cnv A bibliography on copy number variation DEFAULTSORT Array Comparative Genomic Hybridization ... more details
Image CGH schema.jpg right thumb Comparative genomic hybridization CGH or Chromosomal Microarray Analysis CMA is a molecular cytogenetic method for the analysis of copy number variation copy number changes gains losses in the DNA content of a given subject s DNA and often in tumor cell biology cells . CGH will detect only unbalanced chromosomal changes. Structural chromosome aberrations such as balanced reciprocal Chromosomal translocation translocations or Chromosomal inversion inversions cannot be detected, as they do not change the copy number. During the 1990s Thomas Cremer realized together with Peter Lichter the concept of comparative genomic hybridization to metaphase chromosomes and to a matrix with DNA spots representing specific genomic sites. Method DNA from subject tissue and from normal control tissue reference are each labeled with different tags for later analysis by fluorescence. After mixing subject and reference DNA along with unlabeled human cot 1 DNA placental DNA that is enriched for repetitive DNA sequences such as the Alu sequence Alu and Kpn family ref http products.invitrogen.com ivgn en US adirect invitrogen?cmd catProductDetail&productID 15279011 Invitrogen Corporation Human Cot 1 DNA Retrieved on September 9, 2009 ref to suppress repetitive DNA sequences, the mix is Nucleic acid hybridisation Hybridization hybridized to normal metaphase chromosomes or, for Array comparative genomic hybridization array or matrix CGH , to a slide containing hundreds or thousands of defined DNA probes. Using epifluorescence microscopy and quantitative image analysis ... chromosome BAC arrays offering resolution of 100kb. Uses Cancer The method is based on the hybridization ... embryos by metaphase comparative genomic hybridization mCGH improves efficiency of IVF by increasing ... genomic hybridization array CGH overcomes many of these limitations, with improvement in resolution ... hybridization Array based Comparative Genomic Hybridization Oncogene Tumor suppressor gene Carcinoma ... more details
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