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Encyclopedia results for Primers

  1. Oxford Chemistry Primers

    The Oxford Chemistry Primers are a series of short texts providing accounts of a range of essential topics in chemistry and chemical engineering written for undergraduate study. The first primer Organic Synthesis The Roles of Boron and Silicon was published by Oxford University Press in 1991 ref http www.oup.com uk catalogue ?ci 9780198556626 OUP website ref . As of 2010 there are 92 ref name titlelist cite web url http ukcatalogue.oup.com nav p category academic series chemistry ocp.do?sortby bookTitleAscend&thumbby crawl 10&thumbby all title A Z list of OCP Titles accessdate 8 August 2010 ref titles in print, written by a wide range of authors. The editors are Stephen G. Davies Steve G. Davies Organic Chemistry , Richard G. Compton Physical Chemistry , John Evans Inorganic Chemistry and L.F. Gladden Chemical Engineering ref http www.oup.co.uk oxfordtextbooks chemistry ocp OUP website ref . References references Category Chemistry books Category Oxford University Press books Chemistry Primers, Oxford science book stub ...   more details



  1. Stain blocking primers

    Multiple issues wikify January 2011 orphan January 2011 cleanup April 2009 Stain blocking primers are used to cover stains such as watermarks, nicotine, markers, smoke, and prevent them bleeding through newly applied layers of paint. They also provide adhesion over problematic surfaces, giving better film leveling, and durability. Commonly used stain blocking paints include acrylic and alkyd. VOCs Low volatile organic compound s VOC formulations partially or completely eliminates odor, making them safer for the environment. Citation needed date January 2011 However, in the United States, solvent based products with high VOC levels still represent approximately 25 of the total market volume for interior stain blocking primers. They continue to maintain this significant market share even though many national, regional or local legislations and initiatives concerning the reduction of VOCs have been recently established. ref 1. Eliokem Tech Note TN PLW 2006 04 0 Plioway Ultra 350 & Plioway Ultra G 20 in Odorless Interior Stain Blocking Primer ref Since their introduction to the US market in 1997, low VOC, odorless stain blocking primers have become known for their unique combination of highly effective stain blocking. These properties are traditionally associated with solvent based ... primers, sealers and undercoats category. As state regulatory agencies continue to introduce ... based stain blocking primers. In order to decrease the VOC of a coating, it is necessary to remove ... indicate that the use of mineral spirits based primers would need 6 to 7 air changes per hour requiring significant ventilation. The use of low odor, isoparaffinic solvent based primers would only need 1 to 2 air changes per hour. Finally, the use of water based primers would need less than 1 air ... blocking primers with low odor, isoparaffinic solvent are considered to have less of a negative impact ... reflist DEFAULTSORT Stain Blocking Primers Category Paints ...   more details



  1. Tubes and primers for ammunition

    one source date February 2012 no footnotes date February 2012 Tubes and primers are used to ignite the propellant in projectile weapons. In ancient times various devices were adopted to ignition system ignite the Explosive material charge . Small gun s were fired by priming powder poured down the touch hole or vent and ignited by glowing embers or a red hot iron rod. Later the priming powder was ignited by a piece of slow match held in a linstock a stick with a clamp at one end . About 1700, this was effected by means of a port fire. This was a paper case about 16  inch 406  mm long filled with slow burning composition which burnt rather more than one inch 25  mm per minute. Later again the charge was exploded by paper tubes sometimes called Dutch tubes filled with powder and placed in the vent and ignited by a port fire. In comparatively modern times friction tubes have been used, while in the latest patterns percussion or electric tubes are employed. Vent sealing tubes In most breech loading guns it is essential to stop the erosion of the metal of the vent by preventing the escape of gas through it when the gun is fired. For this purpose the charges in such guns are ignited by vent sealing tubes. For muzzle loaded guns and small breech loading guns radially vented, especially those using gunpowder black powder , the amount of erosion in the vent is not so serious. The charge is fired by ordinary friction tubes, which are blown away by the escape of gas through the vent. In all guns axially vented, vent sealing tubes, which are not blown out, must be employed to protect ... tubes In the England English service electric tubes in the United States called primers are mostly ... primers. For small guns the case contains a percussion cap percussion primer , usually a copper ... Commons category Tubes and primers for ammunition Percussion cap Primer firearm Internal priming Internal priming References 1911 DEFAULTSORT Tubes And Primers For Ammunition Category Ammunition ...   more details



  1. Primer (cosmetics)

    primers aid in the smooth application of eye shadow, prevent it from accumulating in eyelid creases, and improve its longevity. There are many different brands of eye shadow primers on the market. Two popular eye shadow primers include Urban Decay Primer Potion and Two Faced Shadow Insurance. Eye shadow primers are applied to the eyelid and lower eye area prior to the application of eye shadow. They even ... shadow primers help with the application of eye shadows. They intensify the color of the eye shadows ... shadow primers is not limited to eye shadows. They also work for eye liners and eye shadow bases. Mascara ..., and some claim to improve the health of the lashes. Lip primers are intended to smooth the lips and help ...   more details



  1. Second Generation Multiplex

    Unreferenced date December 2009 Context date October 2009 Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI Applied Biosystems . It contains primers for the following STR Short Tandem Repeat loci. VWA HUMVWF31 A , D8 D8S1179 , D21 D21S11 , D18 D18S51 , THO HUMTHO1 , FGA HUMFIBRA Also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for detection under electrophoresis. Carboxyfluorescein 5 FAM JOE NED Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM DNA profiling system in 1998 Category Biometrics Category DNA profiling techniques Category Law enforcement in the United Kingdom ...   more details



  1. Nested polymerase chain reaction

    Image Nested PCR.png thumb 250px A diagram illustrating the method of nested PCR. Nested polymerase chain reaction is a modification of polymerase chain reaction intended to reduce the contamination in products due to the amplification of unexpected primer binding sites. Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature mediated DNA polymerase . The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases. Conventional PCR requires primer molecular biology primers complementary to the termini of the target DNA. A commonly occurring problem is primers binding to incorrect regions of the DNA, giving unexpected products. Nested polymerase chain reaction involves two sets of primers, used in two successive runs of polymerase chain reaction, the second set intended to amplify a secondary target within the first run product. Processes The target DNA undergoes the first run of polymerase chain reaction with the first set of primers, shown in green. The selection of alternative and similar primer binding sites gives a selection of products, only one containing the intended sequence. The product from the first reaction undergoes a second run with the second set of primers, shown in red. It is very unlikely that any of the unwanted PCR products contain binding sites for both the new primers, ensuring the product from the second PCR has little contamination from unwanted products of primer dimers, hairpins, and alternative primer target sequences. Resources http www.worldcat.org search?qt affiliate&ai TheWikiLibrarian TheWikiLibrarian&q nested polymerase chain reaction Books your local library about nested polymerase chain reacitons br http scholar.google.com scholar?hl en&lr &q 22Nested polymerase chain reaction 22 Scholarly articles on nested polymerase chain reactions PCR Category Molecu ...   more details



  1. Beacon designer

    Advert date February 2011 No footnotes date February 2011 Infobox Software name Beacon Designer logo screenshot caption developer Premier Biosoft latest release version 7.9 latest release date 17 June 2010 latest preview version latest preview date operating system Windows, Macintosh platform genre Bioinformatics license commercial website http www.premierbiosoft.com Beacon Designer designs highly specific an efficient primers and probes for real time PCR polymerase chain reaction assays. It is compatible to work on Windows as well as on Mac. The software currently supports the following real time PCR chemistries for efficient primer and probe design. 1. SYBR Green br 2. TaqMan br 3. Molecular beacon br 4. HRMA Primers br 5. Scorpions br 6. FRET br 7. Locked nucleic acid LNA Locked nucleic acid br 8. NASBA molecular biology NASBA br Beacon Designer program has built in support for homology avoidance using the standard web BLAST service or the set up of a desktop BLAST feature for identification of regions that are significantly cross homologous with the target gene sequence. The software designs real time pcr primers and probes for complex differential gene expression and allele discrimination assays. Avoidance of the cross homology renders specificity to the primers in question and they are highly targeted and only amplify the intend sequence and no other targets. Beacon Designer has been used in workshops and conferences related to real time PCR study as a reference software. The author company, Premier Biosoft has also launched a simple version for evaluation of oligos for real time PCR primers and probes support limited to TaqMan and SYBR Green primers by the name Beacon designer free version. External links http www.premierbiosoft.com molecular beacons index.html Beacon Designer Homepage http www.premierbiosoft.com qpcr index.html Beacon Designer Free Edition http www.biocompare.com Articles FeaturedArticle 1167 High Resolution Melt.html High Resolution Melt Ca ...   more details



  1. Zerna Sharp

    Unreferenced stub auto yes date December 2009 Zerna Addis Sharp August 12, 1889 June 17, 1981 was an United States U.S. author , writer and teacher. She became known for creating the Dick and Jane Basal reader beginning readers , and many other readers for children. Sharp noted the reduced Reading process reading ability of children during her travels and urged a new reading format for Primer textbook primers . She suggested that primers introduce a word at a time to new readers, and the Dick and Jane primers adhered to this format. The names Dick and Jane were chosen because they were easy to Subvocalization sound . The primers were sold from 1927 to 1973. Sharp was born in Hillisburg , Clinton County, Indiana . She died in Frankfort, Indiana at the age of 91. Persondata Metadata see Wikipedia Persondata . NAME Sharp, Zerna ALTERNATIVE NAMES SHORT DESCRIPTION DATE OF BIRTH August 12, 1889 PLACE OF BIRTH DATE OF DEATH June 17, 1981 PLACE OF DEATH DEFAULTSORT Sharp, Zerna Category American children s writers Category 1889 births Category 1981 deaths Category People from Clinton County, Indiana US child writer stub ...   more details



  1. Primer Premier

    programs that design primers for the PCR reaction. Some of them are listed below 1. Geneious br ...   more details



  1. Primer (paint)

    because it seals the wall and aids in preventing mold . Primers can also be used for dirty surfaces .... Primers can usually be tinted to a close match with the color of the finishing paint. If the finishing ... for good uniformity across the painted surface. Primers for wood There are several reasons ... painting do i need to use paint primer.html archivedate 2008 03 11 ref Quality primers are often .... Some specialty primers are in fact quite costly. Primers are not necessary for a wood stain .... Primers for metal Some metals, such as untreated aluminium, require a primer others may not. A primer ... water seeps through to the bare metal, oxidation will begin plain steel will simply rust . Metal primers ... option, special kinds of primers can be used that chemically convert rust to the solid ... primers and chromating to add the necessary adhesion properties. Primers for plastic Using a primer ... make a thorough bond with the surface being painted. Because most paints and primers designed to be used ...   more details



  1. MassTag-PCR

    Orphan date April 2012 MassTag PCR is a new technology PCR based on Mass spectrometric detector Mass Spectrometric detection of end product. This technology is pioneered by scientist from Center for Infection and Immunity CII of Mailman School of Public Health at Columbia University , USA. Principles Like conventional PCR MassTag PCR also use PCR Primer primer pairs . The difference is primers used for MassTag PCR are tagged with molecules of known masses or MassCodes. Instead of single pair of primers this technology uses a number of primers, making it a multiplex system. Unlike, conventional Multiplex PCR system, in MassTag PCR more than 15 primer pairs could be used. If DNA from any of the agent of primer panel is present, it will be amplified. Each amplified product will carry its specific Masscodes. After PCR the PCR product are purified with to remove unbound primers, dNTPs, enzyme and other impurities. Finally, the purified PCR products are subject of Ultra violet UV as the chemical bond with nucleic acid and primers are photolabile. As the Masscodes are liberated from PCR products they are detected with a Mass Spectrometer.Presence of specific MassCode indicates the presence of specific pathogen. ref http www.ncbi.nlm.nih.gov pubmed 15752453 Diagnostic system for rapid and sensitive differential... Emerg Infect Dis. 2005& 93 PubMed result Bot generated title ref ref http www.cdc.gov ncidod EID vol11no02 04 0492.htm Differential Detection of Multiple Respiratory Pathogens CDC EID Bot generated title ref Applications This technique has been found to detect a previously uncharacterized clade of rhinovirus . ref http cii.columbia.edu pdf Multiplex MassTag PCR for respiratory pathogens.pdf doi 10.1016 j.jcv.2008.06.007 Bot generated title ref MassTag PCR is a more comprehensive and sensitive diagnostic technique, CII was able to determine the cause of this illness for 26 out of 79 previously unknown cases. MassTag PCR demonstrated its tripartite value as a tool f ...   more details



  1. Centerfire ammunition

    , economies of scale are achieved through interchangeable primers for similar cartridges. The expensive ... primers Image 45 ACP FMJ SB 3.jpg thumb right The primer of this unfired cartridge has been sealed ... 1981 National Rifle Association p.65 ref Berdan and Boxer cartridge primers are both considered .... Berdan primer Berdan primers are named after their American inventor, Hiram Berdan of New York who ... patent of September 29, 1869, in US patent 82587 . Berdan primers have remained essentially the same functionally to the present day. Berdan primers are similar to the caps used in the caplock system, being small metal cups with pressure sensitive explosive in them. Modern Berdan primers are pressed ... primers are similar to Berdan primers with one major difference, the location of the anvil. In a Boxer ... fired primers vastly easier to remove for re loading the spent primer assembly is removed by pushing ... compound, but automated machinery producing primers by the hundreds of millions has eliminated ... Large top row and small bottom row pistol cartridge Boxer primers. L R fired, unfired, and inside view. The tri lobe object inside the primer is the anvil. Early primers were manufactured with various ... ammunition manufacturers. Boxer primers for the United States market come in different sizes, based on the application. The types sizes of primers are 0.175 4.45  mm diameter small pistol primers ... with heavy firing pin impact. 0.209 5.31  mm diameter primers for shotgun shell s and modern ... cup. 0.210 5.33  mm diameter large rifle primers, and a thinner or softer metal cup large pistol ...  mm diameter .50 BMG primers, used for the .50 Browning Machine Gun cartridge and wildcat cartridge .... Rifle, large and magnum primers increase the ignition energy delivered to the powder, by supplying ... pistol and rifle primers is the thickness of the primer s case since pistol cartridges usually operate ..., while rifle primers are thicker and stronger, requiring a harder impact from the firing pin . ref ...   more details



  1. Primer (molecular biology)

    PCR , require DNA primers. These primers are usually short, oligonucleotide synthesis chemically synthesized ... segments known as Okazaki fragment s. Along the lagging strand s template, primase builds RNA primers in short bursts. DNA polymerases are then able to use the free 3 Hydroxyl OH groups on the RNA primers ... County. Updated Jan., 2008 ref Uses of synthetic primers about the organic chemistry involved Oligonucleotide synthesis possible methods involving primers Nucleic acid methods DNA sequencing is used ... method uses a primer as a start marker for the chain reaction. In polymerase chain reaction PCR , primers are used to determine the DNA fragment to be amplified by the PCR process. The length of primers ... CAB International isbn 978 1 84593 478 1 page 22 pages 415 chapter 1.6.2. Primers development and virus ... Specificity is influenced by the length of the primers and typically primers between 18 24 nucleotides ... segment. It is worth noting that primers are not essentially always necessary for DNA synthesis ... of primers should have similar melting temperatures since annealing in a PCR occurs for both simultaneously .... Primers should not easily anneal with other primers in the mixture either other copies of same ... contaminating the mixture. Primers should also not anneal strongly to themselves, as internal ... Complement Caluclator ref Degenerate primers main Degenerate bases Sometimes degenerate primers are used. These are actually mixtures of similar, but not identical primers. They may be convenient ... similar but not identical. The other use for degenerate primers is when primer design is based on protein ... bases . Use of degenerate primers can greatly reduce the specificity of the PCR amplification. The problem can be partly solved by using touchdown PCR . Degenerate primers are widely used and extremely ... is not available. Usually, degenerate primers are designed by aligning gene sequencing found ... bases. PCR primers are then synthesized as a mixture of primers corresponding to all permutations ...   more details



  1. Hexamer

    A hexamer is a thing composed out of six sub unit s. In microbiology, a hexamer is one of the proteins composing the polyhedral protein shell that encloses the bacterial micro compartments known as carboxysome carboxysomes . A random hexamer or random hexonucleotides are primers used for various PCR applications such as rolling circle amplification to prime the DNA synthesis. See also commonscat Hexamers wiktionary Hexamer Hexameron Hexa , a prefix mer , an affix chemistry stub Category Oligomers cs Hexamer ...   more details



  1. RNTP

    Unreferenced date December 2006 Orphan date February 2009 A ribonucleotide tri phosphate rNTP is a ribonucleotide with 3 phosphate groups. rNTPs are the building blocks of RNA Synthesis RNA synthesis as well as the synthesis of primers in DNA replication . They also serve as stores of chemical energy, chiefly Adenosine triphosphate ATP . Nucleic acids DEFAULTSORT Rntp Category RNA Biochem stub ...   more details



  1. Lawrence Pellet Primer System

    The Lawrence Pellet Primer System was a development of the Sharps rifle , which was discontinued in 1863. The System was designed to mechanically place the explosive primer which ignites the black powder of the rifle. This feature was to be used when a high rate of fire was needed, or when the weather was too cold to manually place individual primers on the firearm. External links Image at http www.berdansharpshooters.com sharps.html BerdanSharpshooters.com Category Firearm terminology ...   more details



  1. RAPD

    by using random primers. Due to problems in experiment reproducibility, many scientific ... the identical 10 mer primers will or will not amplify a segment of DNA, depending on positions that are complementary to the primers sequence. For example, no fragment is produced if primers annealed too far apart or 3 ends of the primers are not facing each other. Therefore, if a mutation has occurred ... and sequenced. New longer and specific primers are designed for the DNA sequence, which is called ...   more details



  1. FastPCR

    of potential mismatching location of the specified primers or Hybridization probe probes . The in silico ... biology Primers Hybridization probe probes are analyzed for all primer secondary structures including ...   more details



  1. DNA shuffling

    DNA shuffling is a way to rapidly propagate beneficial mutation s in a directed evolution experiment. It is used to rapidly increase DNA library size. ref cite journal last Cohen first J. title How DNA Shuffling Works journal Science volume 293 issue 5528 pages 237 237 doi 10.1126 science.293.5528.237 accessdate 8 May 2011 ref Procedure DNAse is firstly used to fragment a set of parent gene s into pieces of 50 100 base pair bp in length. This is then followed by a polymerase chain reaction PCR without primers DNA fragments with sufficient overlapping homologous sequence will anneal to each other and are then extended by DNA polymerase . Several rounds of this PCR extension are allowed to occur, after some of the DNA molecules reach the size of the parental genes. These genes can then be amplified with another PCR, this time with the addition of Primer molecular biology primers that are designed to complement the ends of the strands. The primers may have additional sequences added to their 5 ends, such as sequences for restriction enzyme recognition sites needed for ligation into a cloning vector. It is possible to recombination recombine portion of these genes to generate hybrids or Chimera genetics chimeric forms with unique properties, this is called DNA shuffling. Shuffling methods Using restriction enzymes Restriction enzyme s that cut in similar places are used to digest members of the gene family DNA fragments are joined together with DNA ligase Large numbers of hybrid biology hybrids are produced which can be tested for unique properties Using DNAse 1 Different members of the gene family are fragmented using DNAse 1 followed by PCR During PCR different members of the family are cross primed, DNA fragments with high homology biology homology will anneal to each other The generated hybrids are then used to generate a DNA library library of mutants which are tested for unique properties See also SCOPE protein engineering RACHITT Homologous recombination Referenc ...   more details



  1. Netprimer

    Orphan date February 2009 Infobox Software name NetPrimer logo screenshot caption developer Premier Biosoft latest release version 1.10 latest release date 6 May 2002 latest preview version latest preview date operating system Windows platform genre Bioinformatics license commercial website http www.premierbiosoft.com netprimer index.html br NetPrimer is a free web based tool used for analyzing primers used in PCR to amplify a DNA sequence ref http www.ebd.csic.es andy MolEcolNotes05.pdf ref . The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature Tm is one of the most important factors that governs the success of a PCR reaction. ref http books.google.com books?id IbAcGxyD06MC&pg PA61&lpg PA61&dq primer melting temperature success of a PCR reaction.&source web&ots B6JffCkBdE&sig gs943CUvnrAT8uo6AbnZewnki44&hl en&sa X&oi book result&resnum 2&ct result ref br NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins, self and cross dimers, runs and repeats. ref pga.mgh.harvard.edu primerbank help.html ref These structures significantly affect the primer efficiency and therefore the success of a PCR reaction. NetPrimer can be used to determine the best primer pairs for a given set of experimental conditions. The program assigns a rating to each primer analyzed ref http www.aphis.usda.gov wildlife damage nwrc publications 08pubs piaggio081.pdf ref . The rating is based on the proximity of the thermodynamic parameters to their ideal scores. In addition to the primer quality, its molecular weight and optical activity both in nmol A260 & g A260 are also presented for quantitation. Primers are analyzed for their GC Guanine Cytosine content ref http jcm.asm.org cgi reprint 39 11 4119.pdf ref . This important parameter determines their annealing strength. br br References reflist br br External links http www.premierbiosoft.com netp ...   more details



  1. Overlap extension polymerase chain reaction

    size are subject to normal PCR, using the outermost primers used in the initial, mutagenic ...   more details



  1. Touchdown polymerase chain reaction

    Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primer molecular biology primers will avoid amplifying nonspecific sequences. The Annealing biology annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The Denaturation biochemistry melting point of the primer sets the upper limit on annealing temperature. At temperatures just below this point, only very specific base pairing between the primer and the template will occur. At lower temperatures, the primers bind less specifically. Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in early steps of amplification will swamp out any specific sequences because of the exponential growth exponential nature of polymerase amplification. The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures. The annealing temperature is decreased in increments for every subsequent set of cycles the number of individual cycles and increments of temperature decrease is chosen by the experimenter . The primer will anneal at the highest temperature which is least permissive of nonspecific binding that it is able to tolerate. Thus, the first sequence amplified is the one between the regions of greatest primer specificity it is most likely that this is the sequence of interest. These fragments will be further amplified during subsequent rounds at lower temperatures, and will out compete the nonspecific sequences to which the primers may bind at those lower temperatures. If the primer initially during the higher temperature phases binds to the sequence of interest, subsequent rounds of polymerase chain reaction can be performed upon the product to further amplify those fragments. References cite journal author Don R, Cox P, Wainwright B, Baker K, Mattick J title Touchdown PCR to circumvent spurious pr ...   more details



  1. Inverse polymerase chain reaction

    no footnotes date April 2012 Image Inverse PCR.png thumb right 250px Summary of the inverse PCR process. Inverse polymerase chain reaction Inverse PCR is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primer molecular biology primers complementary to both Directionality molecular biology termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is available from which primers may be designed. Inverse PCR is especially useful for the determination of Insert molecular biology insert locations. For example, various retrovirus es and transposon s randomly integrate into genomic DNA . To identify the sites where they have entered, the known, internal viral or transposon sequences can be used to design primers that will amplify a small portion of the flanking, external genomic DNA. The amplified product can then be sequenced and BLAST compared with DNA databases to locate the sequence which has been disrupted. The inverse PCR method involves a series of restriction digest s and DNA ligase ligation , resulting in a looped fragment that can be primed for PCR from a single section of known sequence. Then, like other polymerase chain reaction processes, the DNA is amplified by the temperature sensitive DNA polymerase A target region with an internal section of known sequence and unknown flanking regions is identified Genomic DNA is digested into fragments of a few kilobase s by a usually low moderate frequency 6 8 base cutting restriction enzyme . Under low DNA concentrations, self ligation is induced to give a circular DNA product. PCR is carried out as usual, with primers complementary to sections of the known internal sequence. Finally the sequence is compared with the sequence available in the data base. Note although the figure suggests that the circularized ligation product is digested prior to PCR, this is not the case. PC ...   more details



  1. Primou

    Orphan date February 2009 PRIMOU is an enhancement of the PRIMO software program developed at the Genome Center at University of Texas Southwestern Medical Center at Dallas to choose primer molecular biology primers and to calculate their annealing temperature according to the base stacking method. It has been modified by the University of Oklahoma to fix memory leak s and handle multi megabase projects. It is currently maintained by Bruce Roe, and Steve Kenton at the University of Oklahoma . External links http www.genome.ou.edu informatics primou.html Home Page ftp ftp.genome.ou.edu pub programs primou src.tar Download Category Laboratory software ...   more details



  1. Conversion coating

    unreferenced date December 2008 Conversion coatings are coatings for metals where the part surface is converted into the coating with a chemical or electro chemical process. Examples include chromate conversion coating s, phosphate conversion coating s, Bluing steel bluing , black oxide coatings on steel, and anodizing . They are used for corrosion protection, increased surface hardness, to add decorative color and as paint primers. Conversion coatings may be very thin, on the order of 0.00001 . Thick coatings, up to 0.002 , are usually built up on aluminium alloys, either by anodizing or chromate conversion. Category Coatings industry stub es Recubrimiento por conversi n ja ...   more details




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