bare urls date July 2011 Orphan date June 2011 Massive Parallel Sequencing is a term used to describe several revolutionary approaches to DNA sequencing , the so called next generation sequencing NGS technologies or second generation sequencing . These sequencing technologies have emerged in late 1996 ... cost DNA sequencing method based on a new 2 dimensional DNA auto patterning process ,P. Mayer et al., presented at the Fifth International Automation in Mapping and DNA Sequencing Conference, St ... available Next generation sequencing NGS platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated ... parallel manner. This design is a paradigm shift from that of Sanger sequencing , also known as capillary sequencing or first generation sequencing which is based on the electrophoretic separation of chain termination products produced in individual sequencing reactions. ref Next Generation Sequencing ... generation sequencing platforms differ from traditional Sanger sequencing technology in a number of ways. First, the DNA sequencing libraries are clonally amplified in vitro , obviating the need for time ... high throughput sequencing platforms, each utilizes a slightly different strategies. ref Next Generation DNA Sequencing and the Future of Genomic Medicine, Matthew W. Anderson 1,2 and Iris Schrijver 1,2,3, , Genes 2010, 1, 38 69 ref As a massively parallel process, Next generation sequencing NGS generates ... acid composition is decreasing substantially and enabling change in genome sequencing approaches .... Massively Parallel Sequencing The Next Big Thing in Genetic Medicine Am J Hum Genet. 2009 Aug ... would decrease the sequencing cost further and eventually achieve the ultimate goal of 1000 per genome sequencing ,. ref Von BubnoffA 2008 Next generation sequencing the race is on.Cell 132 721 723 ref ref http www.genome.gov 27527585 ref Currently, 5 massively parallel sequencing platforms are commercially ... more details
Biotechnol . 1998, 16, 158 162. PMID http www.ncbi.nlm.nih.gov sites entrez 9586238 ref Sequencing of DNA has largely been based on DNA sequencing dideoxy chain termination developed by Frederick Sanger Sanger et al. ref F. Sanger, S. Nicklen, and A. R. Coulson. DNA sequencing with chain terminating ... to be made to this method. In particular, the incorporation of technological innovation, making sequencing ... Human Genome Sequencing Consortium. Initial sequencing and analysis of the human genome. Nature 409, 860 921 15 February 2001 ref . Briefly, in its modern inception, high throughput genome sequencing also referred to as Shotgun sequencing Whole Genome Shot gun Sequencing involves fragmenting ... of the Human Genome Project. In particular Massively Parallel Sequencing MPS approaches such as those ... to be attractive tools for sequencing. Typically, MPS methods can only obtain short read lengths ... in terms of sequencing repetitive regions of the genome. A challenge of short read sequence data is particularly an issue in sequencing new genomes de novo and in sequencing highly rearranged genome ... variation. ref name marra Microfluidic Sanger Sequencing Microfluidic Sanger sequencing is a lab on a chip application for DNA sequencing, in which the Sanger sequencing steps thermal cycling, sample ... and automating the Sanger sequencing steps. Applications of Microfluidic Sequencing Technologies Other useful applications of DNA sequencing include single nucleotide polymorphism SNP detection, Single ... ref name kan Kan, C., Fredlake, C. P., Doherty, E. A. S. and Barron, A. E. DNA sequencing and genotyping ... design Image with unknown copyright status removed Image Figure Microfluidic sequencing bioprocessor3.png thumbnail 500px right A Microfluidic Sanger sequencing chip layout. This picture was adapted from the design of a microfluidic sequencing platform developed by Richard Mathies University of California ... sequencing chip developed by Richard Mathies and colleagues University of California, Berkeley ... more details
DNA sequencing theory is the broad body of work that attempts to lay analytical foundations for DNA sequencing . The practical aspects revolve around designing and optimizing sequencing projects known ... various sequencing methods to one another. In this sense, it could be considered a branch of systems ... numerical calculations are often conducted for particular problems too. DNA sequencing theory addresses physical processes related to sequencing DNA and should not be confused with theories of analyzing ..., but the latter are primarily concerned with algorithm ic issues. Sequencing as a covering problem All mainstream methods of DNA sequencing rely on reading small fragments of DNA and subsequently ... characteristic of DNA sequencing . Stevens configuration is one such example. ref name stevens cite ... also do not account for factors that are actually important in sequencing, for instance detectable overlap in sequencing fragments, double stranding, edge effects, and target multiplicity. Consequently, development of sequencing theory has proceeded more according to the philosophy of applied ... that this presumes math L ll G math , which is valid for many, though not all sequencing scenarios ... they focused on the so called Gene mapping mapping problem , the abstraction to sequencing is much ... the earliest days of large scale genome sequencing. ref name fleischmann cite journal journal Science volume 269 pages 496 512 year 1995 author Fleischmann, R.D. et al. title Whole Genome Random Sequencing ... to play an important role in DNA sequencing. Ultimately, the main goal of a sequencing project is to close all gaps, so the gap perspective was a logical basis of developing a sequencing model ... to this theory, enabling it to be applied to sequencing projects in which the goal was to completely ... Gap Model for Bacterial Artificial Chromosome Clone Fingerprint Mapping and Shotgun Sequencing ... coverage mapping or sequencing projects. As sequencing projects ramped up in the 1990s ... more details
Orphan date April 2012 File TSMS DNA Preperation 7.jpg thumb 300px Single molecule fluorescent sequencing is one of massively parallel DNA sequencing, which has revolutionized many fields of biology by allowing ... based sequencing systems, ref Metzker, M.L. 2010. Sequencing technologies The next generation. Nat. Rev. Genet. 11 31 46. ref but single molecule, non amplification based sequencing approaches are now ..., N.F., and Quake, S.R. 2009. Single molecule sequencing of an individual human genome. Nat. Biotechnol ... in the HeliScopeTM Sequencing System along with all the reagents necessary for sequencing by synthesis and imaging. The Sequencing System is then allowed to sequence as long as necessary with images ... Sequencing with a HeliScope Genetic Analysis System. Curr Protoc Mol Biol. Chapter 7 Unit7.10. ref How Fluorescent Sequencing Works Sample Preparation I Shearing File TSMS DNA Preperation 1.jpg thumb 300px The Helicos Genetic Analysis System is capable of sequencing nucleic acids over a very broad ... on the number of 3 end hydroxyl groups , and thus having relatively short templates for sequencing ... must be performed to remove very small fragments or the sequencing yield will be reduced. This is done ... molecular biology primer immobilized on a flow cell for sequencing, so it is usually necessary to generate ... size range of tails. IV Blocking If the tailed DNA targeted for sequencing is hybridized to the flow cell directly after tailing, it would have a free 3 hydroxyl that could be extended in the sequencing .... Thus, prior to sequencing, it is also necessary to block the 3 ends of the molecules to be sequenced ... a 3 phosphate or other modification that prevents extension can be similarly effective. DNA Sequencing ... sequencing is carried out on a glass flow cell with 25 channels for the same or different samples ... carried out at 55 C for 1 hr. II Fill and Lock Generally, samples for sequencing are prepared .... To avoid sequencing the unpaired A residues, a fill and lock treatment is needed. After hybridization ... more details
Cancer genome sequencing is the whole genome sequencing of a single, homogeneous or heterogeneous group ... of the DNA or RNA sequences of cancer cell s . Unlike whole genome WG sequencing which is typically from blood cells, such as J. Craig Venter s ref name Venter Genome and James D. Watson s WG sequencing projects, ref name Watson Genome saliva, epithelial cells or bone cancer genome sequencing involves direct sequencing of primary tumor tissue, adjacent or distal normal tissue, the tumor micro environment such as fibroblast stromal cells, or metastatic tumor sites. Similar to whole genome sequencing ... translocations and fusion gene s. Cancer genome sequencing is not limited to WG sequencing and can also includes exome , transcriptome , and micronome sequencing. These methods can be used to quantify ... Historically, cancer genome sequencing efforts has been divided between transcriptome based sequencing ... , first funded in 2005, focuses on DNA sequencing. It has published a census of gene s causally implicated ... of all these changes can be accomplished through various cancer genome sequencing strategies. The power of cancer genome sequencing lies in the heterogeneity of cancers and patients ... between a cancer subtype in one individual to the another individual. Cancer genome sequencing will allow ... aml paper These cellular factions could only have been identified through cancer genome sequencing, showing the information that sequencing can yield, and the complexity and heterogeneity of a tumor ... cancer characterization in individuals, heavily involving sequencing include the Cancer Genome Project ..., including large scale genome sequencing techniques. Hundreds of samples are being collected, sequenced ..., Genome Sequencing Centers, Proteome Characterization Centers, a Data Coordinating Center, and Genome ... File 2nd Generation Sequencing.png thumb 2nd Generation Sequencing File 3rd Generation Sequencing.png thumb 3rd Generation Sequencing Cancer genome sequencing utilizes the same technology involved ... more details
Unreferenced auto yes date December 2009 Orphan date February 2009 Merge to Proteinsequencing date November 2011 A protein sequencer is a machine that is used to determine the sequence of amino acid s in a protein . They work by tagging and removing one amino acid at a time how? by chemicals? what are these chemicals designed to do , which is analysed and identified. This is done repetitively for the whole polypeptide , until the whole sequence is established. This method has generally been replaced by nucleic acid technology, and it is often easier to identify the sequence of a protein by looking at the DNA that codes for it. Protein methods DEFAULTSORT Protein Sequencer Cell biology stub Category Cell biology ... more details
Technical date August 2009 No footnotes article date February 2011 Massive parallel signature sequencing MPSS is a sequenced based approach that can be used to identify and quantify mRNA transcripts present in a sample similar to serial analysis of gene expression SAGE but the biochemical manipulation and DNA sequencingsequencing approach differ substantially. MPSS allows mRNA transcripts to be identified through the generation of a 17 20 bp base pair signature sequence adjacent to the 3 end of the 3 most site of the designated restriction enzyme commonly Sau3A or DpnII restriction endonuclease family DpnII . Each signature sequence is cloned onto one of a million microbeads . The technique ensures that only one type of DNA sequence is on a microbead. So if there are 50 copies of a specific transcript in the biological sample, these transcripts will be captured onto 50 different microbeads, each bead holding roughly 100,000 amplified copies of the specific signature sequence. The microbeads are then arrayed in a Flow cytometry flow cell for sequencing and quantification. The sequence signatures are deciphered by the parallel identification of four bases by hybridization to fluorescently labeled encoders Figure 5 . Each of the encoders has a unique label which is detected after hybridization by taking an image of the microbead array. The next step is to cleave and remove that set ... Signature Sequencing MPSS Introduction Massively Parallel Signature Sequencing MPSS is an open ... A ends, so the DpnII end is available for the sequencing reactions. Probe Sample Queries Search Text Probes MPSS tag probe type References Reinartz J et al. Massively parallel signature sequencing MPSS ... parallel signature sequencing MPSS on microbead arrays. Nat Biotechnol. 2000 Jun 18 6 630 4. Erratum ... C. Gene expression profiling by massively parallel sequencing. Genome Res. 2007 Nov 21 PMID 18032722 ... Gene expression analysis by massively parallel signature sequencing MPSS on microbead arrays journal ... more details
DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire ... first8 I. last9 Nilsen first9 G. B. title Human Genome Sequencing Using Unchained Base Reads on Self ... nanoball sequencing This DNA sequencing method allows large numbers of DNA nanoballs to be sequenced per run at lower reagent costs compared to other next generation sequencing platforms. ref name genome cite journal last1 Porreca first1 Gregory J title Genome sequencing on nanoballs journal Nature ... uses DNA nanoball sequencing to sequence samples submitted by researchers. Citation needed date March 2011 Procedure DNA Nanoball Sequencing involves isolating DNA that is to be sequenced, shearing ... strand, and are compacted into a DNA nanoball. The nanoballs are then adsorbed onto a sequencing flow cell. Unchained sequencing reactions interrogate specific nucleotide locations in the nanoball by ligating ..., is sonicated to break the DNA double strands at random intervals. Bioinformatic mapping of the sequencing ... Next generation DNA sequencing of paired end tags PET for transcriptome and genome analyses journal ... ref Therefore, selecting the ideal fragment lengths of the DNA for sequencing the fragments are size ... sequencing by ligation File Unchained Ligation Sequencing.png thumb left upright 1.6 Figure 6. Unchained Ligation Sequencing The order of the DNA bases between the adapter sequences is determined ... Wang title State of the art de novo assembly of human genomes from massively parallel sequencing data ... name pmid19892942 Advantages File DNA Nanoball sequencing flow cell.jpg thumb left Figure 8. DNA Nanoball sequencing flow cell top has high density of sequencing reads with most positions occupied compared to other next generation sequencing platforms bottom DNA nanoball sequencing technology offers several advantages over other sequencing platforms. One major advantage is the use of very high density ... Top . ref name pmid19892942 compared to other sequencing arrays where molecules of DNA are added to a flow ... more details
Electrofocusing Protein structures X ray crystallography Protein NMR ProteinProtein interactions Yeast two hybrid system Protein fragment complementation assay Co immunoprecipitation Affinity purification and mass spectrometry Protein DNA interactions ChIP on chip Chip Sequencing DamID Microscale Thermophoresis Other methods Hydrogen deuterium exchange Mass spectrometry Molecular dynamics Protein structure prediction ProteinsequencingProtein structural alignment Protein ontology Protein synthesis ...Protein methods are the techniques used to study protein s. There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified. Genetic methods conceptual translation many proteins are never directly sequenced, but their sequence of amino acids is known by conceptual translation of a known mRNA sequence. See genetic code . site directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function. insertion of protein tags such as the His tag . See also green fluorescent protein . evolutionary analysis of sequence changes in different species using ... . Detecting proteins microscopy, Immunostaining protein immunostaining Immunoprecipitation Protein immunoprecipitation Immunoelectrophoresis Immunoblotting Bicinchoninic acid assay BCA Protein Assay Western blot Spectrophotometry Enzyme assay Protein purification Protein Isolation chromatography ... , Affinity chromatography Protein Extraction and Solubilization Protein Concentration Determination Methods, Bradford protein assay Concentrating Protein Solutions Gel electrophoresis Gel Elecdsis Under ... with DNA or RNA Nucleic acid methods References Protein Methods , 2nd Edition by Daniel M. Bollag ... template. No More Links Protein topics Protein methods Category Protein methods ca M tode proteic ... more details
Primarysources date October 2007 The Baylor College of Medicine Human Genome Sequencing Center BCM HGSC was established by Richard Gibbs biologist Richard A. Gibbs in 1996 when BCM was chosen as one of six worldwide sites to complete the final phase of the International Human Genome Project . Gibbs is the current director of the BCM HGSC. It occupies more than convert 36000 sqft m2 , employs over 200 staff, including eighteen faculty, and is one of three National Institutes of Health funded genome centers that were involved in the completion of the first Human Genome Sequence in 2004. The BCM HGSC contributed approximately 10 percent of the total project by sequencing Chromosomes 3, 12 and X. The BCM HGSC collaborated with researchers at the U.S. Department of Energy s Lawrence Berkeley Laboratory and Celera Genomics to sequence the first species of Drosophilidae fruit fly , Drosophila melanogaster . The BCM HGSC also completed the second species of fruit fly Drosophila pseudoobscura , the honeybee Apis mellifera , and led an international consortium to sequence the Brown Norway rat. The Human Genome Sequencing Center subsequently sequenced and annotated the genome of the cow Bos taurus , the sea urchin , Rhesus macaque, Tammar wallaby , Dictyostelium discoideum , and a number of bacteria that cause serious infection s Rickettsia typhi, Enterococcus faecium, Mannheimia Haemolytica , and Fusobacterium nucleatum . The BCM HGSC was a major contributor to the Mammalian Gene Collection program, to sequence all human cDNAs, as well as the International Haplotype Mapping Project HapMap . Other research within the BCM HGSC includes new molecular technologies for mapping and sequencing, exploration of novel chemistries for DNA tagging, development of instrumentation for DNA manipulation, building new computer programs for genomic data analysis, and studying the genes expressed .... The sequencing for the Drosophila Genetic Reference Panel was performed here. The DGRP is a collaborative ... more details
Refimprove date November 2007 Flu The Influenza Genome Sequencing Project IGSP is an American based genome project aimed at improving the availability of genomic sequence data from influenza virus es and related information. The project is funded by the National Institute of Allergy and Infectious Diseases NIAID , a division of the National Institutes of Health NIH , and has been operating out of the NIAID Microbial Sequencing Center at The Institute for Genomic Research TIGR . Sequence information generated by the project has been placed into public domain through GenBank . Origins In early 2004, David Lipman , Lone Simonsen , Steven Salzberg , and a consortium of other scientists wrote a proposal to begin sequencing large numbers of influenza virus es at The Institute for Genomic Research TIGR . Prior to this project, only a handful of flu genomes were publicly available. Citation needed date October 2008 Their proposal was approved by the National Institutes of Health NIH , and would later become the IGSP. New technology development led by Elodie Ghedin began at TIGR later that year, and the first publication describing 100 influenza genomes appeared in 2005 in the journal Nature ref name Ghedin05 cite journal author Ghedin E, Sengamalay NA, Shumway M, et al. title Large scale sequencing of human influenza reveals the dynamic nature of viral genome evolution journal Nature volume 437 issue 7062 pages 1162 6 year 2005 month October pmid 16208317 doi 10.1038 nature04239 url http www.nature.com nature journal v437 n7062 full nature04239.html laysummary http www.accessexcellence.org WN SU flugenestorynov05.htm More Flu Viruses Around than Expected, Mutate in Unexpected Ways ... Sequencing Project home page at TIGR http www.niaid.nih.gov dmid genomes mscs influenza.htm Influenza Genome Sequencing Project home page at NIAID http www.ncbi.nlm.nih.gov genomes FLU FLU.html Influenza ... projects Category Influenza Genome Sequencing Project ... more details
Wikify date April 2010 Orphan date August 2009 Flats Sequencing System FSS is an automated system used by the US Postal Service . It uses a dual pass sort technique to sort Flats USPS flats all the way to delivery sequence order. Prior to the deployment of FSS machines, flats were machine sorted to the route level only. Carriers had to manually sort all the flats they were to deliver into proper delivery order before they could embark on their routes. With FSS, when the carrier receives them they are ready to be delivered without need for any further manual sorting. FSS machines are manufactured by Northrop Grumman . Deployment began in 2008. External links http www.es.northropgrumman.com solutions flatssequencing assets flatssequencing.pdf manufacturer s PDF product brochure http www.youtube.com watch?v dsRUnKiMQoM Promotional video done in the style of a movie trailer Category United States Postal Service ... more details
Unreferenced date December 2009 Alternate frame sequencing , A hyphen has no grammatical or other justification for being in there. Will someone who has the hang of moving renaming articles and implementing the necessary redirection kindly remove it from the name of the article? also known as alternate image , AI , alternating field , field sequential , etc., uses the eclipse method of displaying stereoscopy stereoscopic 3 D images. It works by openly presenting the image intended for the left eye while blocking the right eye s view, then presenting the right eye image while blocking the left eye, and repeating this so rapidly that the interruptions do not interfere with the perceived fusion of the two images into a single 3 D image. Alternate frame sequencing is used to present 3 D film s in some theaters. It can be used to present 3 D images on CRT , plasma display plasma , LCD and other types of video displays. Although virtually all ordinary unmodified video and computer systems can be used to display 3 D by adding a plug in interface and LCD shutter glasses , disturbing levels of flicker or ghosting may be apparent with systems or displays not designed for such use. The rate of alternation required to completely eliminate noticeable flicker depends on image brightness and other factors, but is typically well over 30 image pair cycles per second, the maximum possible with a 60 Hz display. A 120 Hz display, allowing 60 images per second per eye, is widely accepted as flicker free. Applications in film The principle made its public debut remarkably early. In 1922, the Teleview 3 D system was installed in a single theater in New York City. Several short films and one feature length film were shown by running left eye and right eye prints in a pair of interlocked projectors with their shutters operating out of phase. Each seat in the auditorium was equipped with a viewing ... Frame Sequencing Category 3D imaging it Alternate Image ... more details
Refimprove date February 2007 Sequencing batch reactors SBR or sequential batch reactors are industrial processing tanks for the treatment of wastewater . SBR reactors treat waste water such as sewage or output from anaerobic digester s or mechanical biological treatment facilities in batches. Oxygen is bubbled through the waste water to reduce biochemical oxygen demand BOD and chemical oxygen demand COD to make suitable for discharge into sewers or for use on land. While there are several configurations of SBRs the basic process is similar. The installation consists of at least two identically equipped tanks with a common inlet, which can be switched between them. The tanks have a flow through system, with raw wastewater influent coming in at one end and treated water effluent flowing out the other. While one tank is in settle decant mode the other is aeration aerating and filling. At the inlet is a section of the tank known as the bio selector. This consists of a series of walls or baffles which direct the flow either from side to side of the tank or under and over consecutive baffles. This helps to mix the incoming Influent and the returned activated sludge , beginning the biological digestion process before the liquor enters the main part of the tank. Treatment Stages There are five stages to treatment p 1. Fill p 2. React p 3. Settle p 4. Decant p 5. Idle p The inlet valve opens and the tank is being filled in, while mixing is provided by mechanical means no air . This stage is also called the anoxic stage. p Aeration of the mixed liquor is performed during the second stage by the use of fixed or floating mechanical pumps or by transferring air into fine bubble diffusers fixed to the floor of the tank. p No aeration mixing is provided in the third stage and the settling ... biological treatment sequencing batch reactor sbr Pages jettech product omniflo sbr.aspx Sequencing ... Environmental technology Category Environmental engineering Category Sewerage de Sequencing Batch ... more details
The Sonority Sequencing Principle SSP ref name Selkirk1984 Selkirk, E. 1984 . On the major class features and syllable theory. In Aronoff & Oehrle eds. Language Sound Structure Studies in Phonology. Cambridge MIT Press. 107 136. ref ref name Clements1990 Clements, G. N. 1990 . The role of the sonority cycle in core syllabification. In J. Kingston and M. E. Beckman eds. Papers in Laboratory Phonology I Between the grammar and the physics of speech. Cambridge Cambridge University Press. 283 333. ref is a phonotactic principle that aims to outline the structure of a syllable in terms of sonority . The SSP states that the center of a syllable, namely the syllable nucleus , often a vowel , constitutes a sonority peak that is preceded and or followed by a sequence of segments consonants with progressively decreasing sonority values i.e., the sonority has to fall toward both edges of the syllable . The sonority values of segments are determined by a sonority hierarchy . A good example for the SSP in English language English is the one syllable word trust The first consonant in the syllable onset is t , which is a stop consonant stop , the lowest on the sonority scale next is r , a liquid consonant liquid which is more sonorous, then we have the vowel u IPA en the sonority peak next, in the syllable coda , is s , a fricative , and last is another stop, t . Some languages possess syllables that violate the SSP Russian language Russian and English, for example while other languages strictly adhere to it, even requiring larger intervals on the sonority scale In Italian language Italian for example, a syllable initial stop must be followed by either a liquid, a semivowel glide or a vowel, but not by a fricative except ps borrowed words like pseudonimo, psicologia . Some languages allow a sonority plateau that is, two adjacent tautosyllabic consonants with the same sonority level. Modern Hebrew is an example of such language. Sources references Category Phonology Category Phonotactics ... more details
Proteinprotein interaction prediction is a field combining bioinformatics and structural biology in an attempt ... proteinprotein interaction s is important for the investigation of intracellular signaling pathways, modelling of protein complex structures and for gaining insights into various biochemical ... of experimental techniques, including yeast two hybrid screening two hybrid systems, protein fragment complementation assays PCA , affinity purification mass spectrometry , protein microarray s, fluorescence ... in other organisms. In addition, a number of bound protein complexes have been structurally solved ... pairs of protein families with similar patterns of presence or absence across large numbers of species ... imply physical interaction. Prediction of co evolved protein pairs based on similar phylogenetic ... protein in the hypothesized interacting pair. If the matrices are sufficiently similar as measured ... ref Ogmen builds a library of known proteinprotein interfaces from the Protein Data Bank PDB , where ... a known structure i.e. present in the Protein Data Bank PDB . Bayesian network modelling Bayesian ... potential protein interaction is a true positive result. These methods are useful because ... circumstantial evidence that a particular pair of proteins might interact. 3D template based protein complex modelling This method ref Aloy ref Chen ref Fukuhara ref Kittichotirat makes use of known protein complex structures to predict as well as structurally model interactions between query protein ... to search for protein complex structures that are homologous to the query sequences. These known ... sequences. This method has the advantage of not only inferring protein interactions but also suggests ... is limited to a relatively small number of known protein complex structures. Supervised learning problem ... the known protein interactions supervise the estimation of a function that can predict whether an interaction .... . Relationship to docking methods The field of proteinprotein interaction prediction is closely related ... more details
Orphan date February 2009 PBB geneid 84070 Protein FAM186B is a protein that in humans is encoded by the FAM186B gene . ref name pmid11230166 cite journal author Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Bocher M, Blocker H, Bauersachs S, Blum H, Lauber J, Dusterhoft A, Beyer A, Kohrer K, Strack N, Mewes HW, Ottenwalder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A title Toward a catalog of human genes and proteins sequencing and analysis of 500 novel complete protein coding human cDNAs journal Genome Res volume 11 issue 3 pages 422 35 year 2001 month Mar pmid 11230166 pmc 311072 doi 10.1101 gr.GR1547R ref ref name entrez cite web title Entrez Gene C12orf25 chromosome 12 open reading frame 25 url http www.ncbi.nlm.nih.gov sites entrez?Db gene&Cmd ShowDetailView&TermToSearch 84070 accessdate ref The PBB Summary template is automatically maintained by Protein Box Bot. See Template PBB Controls to Stop updates. PBB Summary section title summary text References reflist Further reading refbegin 2 PBB Further reading citations cite journal author Mehrle A, Rosenfelder H, Schupp I, et al. title The LIFEdb database in 2006. journal Nucleic Acids Res. volume 34 issue Database issue pages D415 8 year 2006 pmid 16381901 doi 10.1093 nar gkj139 pmc 1347501 cite journal author Wiemann S, Arlt D, Huber W, et al. title From ORFeome to biology a functional genomics pipeline. journal Genome Res. volume 14 issue 10B pages 2136 44 year 2004 pmid 15489336 doi 10.1101 gr.2576704 pmc 528930 cite journal author Gerhard DS, Wagner L, Feingold ... sequencing and characterization of 21,243 full length human cDNAs. journal Nat. Genet. volume ... by large scale cDNA sequencing. journal EMBO Rep. volume 1 issue 3 pages 287 92 year 2001 pmid ... The PBB Controls template provides controls for Protein Box Bot, please see Template PBB Controls for details. PBB Controls update page yes require manual inspection no update protein box yes update ... more details
wiktionarypar proteinProtein is an organic compound made of amino acids. Protein may also refer to Protein nutrient Protein band See also List of topics related to protein disambig es Prote na desambiguaci n lv Prote ni noz mju atdal ana ... more details
Protein database may refer to Any protein structure database Any protein sequence database Exact names Protein NIH Protein database of the National Institute of Health Protein Database of Bio Synthesis, Inc. disambig ... more details
A binding protein is any protein that acts as an agent to Molecular binding bind two or more molecules together. Examples include DNA binding protein Single strand binding protein Telomere binding protein RNA binding protein Poly A binding protein Nuclear cap binding protein complex CREB binding protein Calcium binding protein Calcium binding protein 1 S100 calcium binding protein A1 TATA binding protein Actin binding protein Penicillin binding proteins Retinol binding protein Retinol binding protein 4 EP300 Binding immunoglobulin protein Odorant binding protein Lipopolysaccharide binding protein C4b binding protein Rap GTP binding protein Calmodulin binding proteins Iron binding proteins Thyroxine binding proteins Folate binding protein Sterol regulatory element binding protein GTP binding protein Retinaldehyde binding protein 1 Ccaat enhancer binding proteins Androgen binding protein Maltose binding protein Phosphatidylethanolamine binding protein 1 Syntaxin binding protein 3 Insulin like growth factor binding protein Methyl CpG binding domain protein 2 Growth hormone binding protein Vitamin D binding protein Syntaxin binding protein 2 Oxysterol binding protein E3 binding protein Iron responsive element binding protein Polypyrimidine tract binding protein Fatty acid binding protein Myosin binding protein C, cardiac CPE binding protein Category Proteins biochem stub ... more details
Protein supplement may refer to Dietary supplement s Bodybuilding supplement Protein Bodybuilding supplement Protein bar See also Protein Disambig ... more details
Protein K may refer to Protein K porin , a porin expressed in pathogenic strains of E. coli Protein K gene expression , a DNA and RNA binding protein expressed in the nucleus of eukaryotes disambig ... more details
Citrullinated protein may refer to Any protein having undergone citrullination The proteins often cyclic ones being the targets of anti citrullinated protein antibody anti citrullinated protein antibodies disambig ... more details
PBB geneid 387357 Protein THEMIS is a protein that in humans is encoded by the THEMIS gene . ref name pmid19597499 cite journal author Fu G, Vall e S, Rybakin V, McGuire MV, Ampudia J, Brockmeyer C, Salek M, Fallen PR, Hoerter JA, Munshi A, Huang YH, Hu J, Fox HS, Sauer K, Acuto O, Gascoigne NR title Themis controls thymocyte selection through regulation of T cell antigen receptor mediated signaling journal Nat. Immunol. volume 10 issue 8 pages 848 56 year 2009 month August pmid 19597499 doi 10.1038 ni.1766 url issn ref ref name pmid19597498 cite journal author Lesourne R, Uehara S, Lee J, Song KD, Li L, Pinkhasov J, Zhang Y, Weng NP, Wildt KF, Wang L, Bosselut R, Love PE title Themis, a T cell specific protein important for late thymocyte development journal Nat. Immunol. volume 10 issue 8 pages 840 7 year 2009 month August pmid 19597498 doi 10.1038 ni.1768 url issn ref ref name pmid19597497 cite journal author Johnson AL, Aravind L, Shulzhenko N, Morgun A, Choi SY, Crockford TL, Lambe T, Domaschenz H, Kucharska EM, Zheng L, Vinuesa CG, Lenardo MJ, Goodnow CC, Cornall RJ, Schwartz RH title Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection journal Nat. Immunol. volume 10 issue 8 pages 831 9 year 2009 month August pmid 19597497 doi 10.1038 ni.1769 url issn ref Function This protein plays a regulatory role in both positive and negative T cell selection during late thymocyte development. The protein functions through T cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T cells. ref name entrez cite web title Entrez Gene thymocyte selection associated url http www.ncbi.nlm.nih.gov ... who weighed the fates of humans, an apt choice since protein is important in deciding the fate ... T, et al. title Complete sequencing and characterization of 21,243 full length human cDNAs. journal ... protein expression libraries using random activation of gene expression. journal Nat. Biotechnol. volume ... more details
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