refimprove date August 2008 Orphan date November 2006 A proteose is any of various water soluble Chemical compound compounds that are produced during digestion by the hydrolytic breakdown of proteins short of the amino acid stage. Category Proteins protein stub zh ... more details
Hoyle s Agar is a Growth medium Selective media selective media that uses tellurite to differentially select Corynebacterium diphtheriae from other upper respiratory tract flora. Hoyle s tellurite agar contains class wikitable Peptone Peptide classes Proteose Peptone 10 g L Beef extract 10 g L Sodium chloride 5 g L Blood, laked tellurite 0.35 g L Agar 15 g L Growth media Category Microbiological media Microbiology stub ar pl Po ywka Clauberga ... more details
XLT Agar Xylose Lysine Tergitol 4 Is a selective culture medium for the isolation and identification of salmonella e from food and environmental samples. It is similar to XLD agar however the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus bacterium Proteus spp. and other non Salmonellae. ref Cite pmid 7790441 ref Successful growth of Salmonella will result in growth of red colonies with a black centre. XLT Agar contains class wikitable Peptone Peptide classes Proteose Peptone 1.6g l Yeast extract 3g l L Lysine 5g l Xylose 3.75g l Lactose 7.5g l Sucrose 7.5g l Sodium chloride 5g l Sodium thiosulphate 6.8g l Ferric ammonium citrate 0.8 mg l Phenol red 0.08g l Agar 18g l See also agar plate XLD agar R2a agar MRS agar References See http en.wikipedia.org wiki Wikipedia Footnotes for a discussion of different citation methods and how to generate footnotes using the ref , ref and reference tags Reflist External links http www.foodnavigator.com news ng.asp?id 63459 salmonella Growth media DEFAULTSORT Xlt Agar Category Microbiological media microbiology stub th XLT agar ... more details
R2A agar is a culture medium ref name pmid10528669 cite journal author van der Linde K, Lim BT, Rondeel JM, Antonissen LP, de Jong GM title Improved bacteriological surveillance of haemodialysis fluids a comparison between Tryptic soy agar and Reasoner s 2A media journal Nephrol. Dial. Transplant. volume 14 issue 10 pages 2433 7 year 1999 month October pmid 10528669 doi 10.1093 ndt 14.10.2433 url http ndt.oxfordjournals.org cgi pmidlookup?view long&pmid 10528669 ref developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow growing species and would quickly be suppressed by faster growing species on a richer culture medium. Since its development in 1979, ref name pmid42349 cite journal author Reasoner DJ, Blannon JC, Geldreich EE title Rapid seven hour fecal coliform test journal Appl. Environ. Microbiol. volume 38 issue 2 pages 229 36 year 1979 month August pmid 42349 pmc 243471 doi url http aem.asm.org cgi pmidlookup?view long&pmid 42349 ref it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. Typical Composition g l Proteose peptone, 0.5 br Casamino acids , 0.5 br Yeast extract , 0.5 br Dextrose , 0.5 br Soluble starch , 0.5 br Dipotassium phosphate, 0.3 br Magnesium sulfate 7H sub 2 sub O, 0.05 br Sodium pyruvate , 0.3 br Agar , 15 br Final pH 7.2 0.2 25 C References reflist Category Growth media Growth media ... more details
Wikify date June 2011 Thiosulfate citrate bile salts sucrose agar or TCBS agar is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio spp. ref cite journal last1 Pfeffer first1 C. last2 Oliver first2 J.D. year 2003 title A comparison of thiosulphate citrate bile salts sucrose TCBS agar and thiosulphate chloride iodide TCI agar for the isolation of Vibrio species from estuarine environments journal Letters in Applied Microbiology volume 36 pmid 12581373 issue 3 pages 150 151 url doi 10.1046 j.1472 765X.2003.01280.x ref TCBS Agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other vibrios. Inhibition of gram positive bacteria is achieved by the incorporation of ox gall , which is a naturally occurring substance containing a mixture of bile salts, and sodium cholate , a pure bile salt . Sodium thiosulfate serves as a sulfur source and, in combination with ferric citrate, detects hydrogen sulfide production. Saccharose sucrose is included as a Fermentation biochemistry fermentable carbohydrate for the metabolism of vibrios. The alkaline pH of the medium enhances the recovery of V. cholerae. Thymol blue and bromothymol blue are included as pH indicator indicators of pH changes. Formula See talk page re. inconsistency Approximate formula per liter Yeast extract 5.0g Peptide Proteose Peptone 10.0g Sodium citrate 10.0g Sodium thiosulfate 10.0g Ox gall 8.0g Saccharose 20.0g Sodium chloride 10.0g Ferric ammonium citrate 1.0g Bromothymol blue 0.04g Thymol blue 0.04g Agar 15.0g Expected results Typical colonial morphology on TCBS Agar is as follows V. cholerae ........................... Large yellow colonies. V. parahaemolyticus ............. Colonies with blue to green centers. V. alginolyticus ..................... Large yellow mucoidal colonies. V. harvey V. fishceri ..............Greyish green to bluish green colonies which show luminiscence in dark. Older colonies fail to show biolumi ... more details
Glucose Phosphate Broth br It is used to perform Methyl red Methyl Red MR test and Voges Proskauer test Voges Proskauer VP test. Contents Glucose 5 g l br Dipotassium phosphate 5 g l br Proteose Peptone 5 g l br Distilled water 1000 ml br pH 6.9 br Methyl Red test br Principle It is used to determine the ability of an organism to produce Mixed acid fermentation mixed acids by fermentation of glucose and to overcome the buffering capacity of the medium. Procedure Inoculate MR VP Glucose phosphate broth with pure culture of test organism. Incubate the broth at 35 sup 0 sup C for 48 72 hours. After incubation add 5 drops of Methyl Red directly into the broth, through the sides of the tube. br Interpretation The development of stable red color in the surface of the medium indicates sufficient acid production to lower the pH to 4.4 and constitute a positive test. Since other organism may produce lesser quantities of acid from the test substrate , an intermediate orange color between yellow and red may develop. This does not indicate positive test. br Controls br Positive and negative controls should be run after preparation of each lot of medium. br Positive control Escherichia coli br Negative control Klebsiella br br Voges Proskauer test br Principle br It is used to determine the ability of some organisms to produce a neutral end product, acetyl methyl carbinol acetoin from glucose fermentation.The production of acetoin, a neutral reacting end product produced by members such as Klebsiella, Enterobacter etc., is the chief end product of glucose metabolism and form less quantities of mixed acids. In the presence of atmospheric oxygen and 40 KOH, acetoin is converted to diacetyl and Naphthol serves as catalyst to bring out red color complex. Media Glucose Phosphate Broth Reagents A Naphthol 5 gm Absolute Ethyl Alcohol 100 ml 0.6 ml 3 parts B KOH 40 g Distilled water 100 ml 0.2 ml 1 part Procedure Inoculate a tube of glucose phosphate broth with a pure inoculum of te ... more details
Image MacConkey agar with LF and LF colonies.jpg thumb right Macconkey agar with lactose left and non lactose right fermenters Image Proteus McConkey.jpg thumb right A MacConkey agar plate with an active bacterial culture. Image Lactose3.png thumb Lactose Alfred Theodore MacConkey MacConkey agar is a Microbiological culture culture Selective medium medium designed to grow Gram negative bacteria and Staining stain them for lactose industrial fermentation fermentation . ref name url cite web url http medic.med.uth.tmc.edu path macconk.htm title tmc.edu work accessdate ref Contents It contains bile salts to inhibit most Gram positive bacteria, except Enterococcus and some species of Staphylococcus i.e. Staphylococcus aureus , crystal violet dye which also inhibits certain Gram positive bacteria , neutral red dye which stains microbes fermenting lactose , lactose and peptone . Composition ref http www.microbelibrary.org index.php component resource laboratory test 2855 macconkey agar plates protocols ref Peptone 17 g Proteose peptone 3 g Lactose 10 g Bile salts 1.5 g Sodium chloride 5 g Neutral red 0.03 g Agar 13.5 g Water add to make 1 litre adjust pH to 7.1 0.2 There are many variations of MacConkey agar depending on the need. If the spreading or swarming of Proteus species is NOT required, sodium chloride is omitted. Crystal violet at a concentration of 0.0001 0.001 g per litre is included when needing to check if Gram positive bacteria are inhibited. History The medium was developed by Alfred Theodore MacConkey while working as a Bacteriology bacteriologist for the Royal Commission on Sewage Disposal. Uses Acting as a visual pH indicator, the agar distinguishes those Gram negative bacteria that can ferment the sugar lactose Lac from those that cannot Lac . This medium is also known as an indicator medium and a low selective medium . Absence of electrolytes serves to inhibit swarming by Proteus species. Lac By utilizing the lactose available in the medium, Lac bacter ... more details
Encyclopedia
top
