Recombinant may refer to A recombinant organism an organism that contains a different combination of Allele alleles from either of its parents. Recombinant DNA a form of artificial DNA Recombinant virus a virus formed by recombining genetic material VRLA , a valve regulated lead acid VRLA battery that is also referred to as a recombinant battery InSoc Recombinant , an album by synthpop band Information Society See also Recombination disambiguation disambig de Rekombinant ... more details
Recombinant DNA rDNA molecules are DNA sequences that result from the use of laboratory methods molecular ... that would not otherwise be found in biological organisms. Recombinant DNA is possible because DNA ... DNA is replicated along with the host DNA. Introduction Recombinant DNA molecules are sometimes ... to the production of sticky and blunt ends . The DNA sequences used in the construction of recombinant ... into recombinant molecules. Using recombinant DNA technology and synthetic DNA, literally any ... that result from the expression of recombinant DNA within living cells are termed List of recombinant proteins recombinant proteins . When recombinant DNA encoding a protein is introduced into a host organism, the recombinant protein will not necessarily be produced Citation needed date September ... 2011 Recombinant DNA differs from genetic recombination in that the former results from artificial ... of existing DNA sequences in essentially all organisms. Creating recombinant DNA main Molecular cloning Image recombinant formation of plasmids.svg thumb 280px right Construction of recombinant DNA ... is the laboratory process used to create recombinant DNA. ref name isbn0 201 75054 6 cite book author ... book author Watson, James D. title Recombinant DNA Genes and Genomes A Short Course publisher W.H. ... PCR replicates DNA in the test tube, free of living cells. Formation of recombinant DNA requires ... in inserting foreign DNA, identifying cells that contain recombinant DNA, and, where appropriate ... Creation of recombinant DNA, 5 Introduction of recombinant DNA into the host organism, 6 Selection of organisms containing recombinant DNA, 7 Screening for clones with desired DNA inserts and biological ... molecular cloning . Expression of recombinant DNA main Gene expression Following transplantation into the host organism, the foreign DNA contained within the recombinant DNA construct may or may ... be Transcription genetics transcribed and Translation biology translated so that a recombinant ... more details
A recombinant virus is a virus produced by recombining pieces of DNA using recombinant DNA technology. This may be used to produce vaccine viral vaccine s or gene therapy vectors. It is also used to refer to naturally occurring recombination between virus genomes in a cell infected by more than one virus strain. This occurs either by homologous crossing over of the nucleic acid strands or by reassortment of genomic segments. Both these and mutation within the virus have been suggested as ways in which influenza and other viruses evolve. An example of a recombinant virus is Western equine encephalitis virus WEE , which is a recombinant virus between two other closely related yet distinct encephalitis viruses. In addition, reassortment is most important for pandemic influenza viruses. See also Reassortment Mutation References http www.cdc.gov ncidod eid vol10no4 03 0396.htm Recombination Resulting in Virulence Shift in Avian Influenza Outbreak, Chile. Suarez et al 2009 http mbe.oxfordjournals.org cgi content abstract 26 1 177?maxtoshow &HITS 1&hits 1&RESULTFORMAT &andorexacttitle phrase&titleabstract H5N1&andorexacttitleabs and&andorexactfulltext and&searchid 1&usestrictdates yes&resourcetype HWCIT&ct Homologous Recombination as an Evolutionary Force in the Avian Influenza A Virus. He at al 2008 External links http www.pbs.org wgbh amex influenza sfeature drjeffrey8.html Dr. Jeffery Taubenberger on A Recombinant Virus http pathmicro.med.sc.edu mhunt genet.htm Viral Genetics http www.cdc.gov flu avian gen info transmission.htm Transmission of Influenza A Viruses Between Animals and People Virus topics DEFAULTSORT Recombinant Virus Category Virology Category Influenza ... more details
Infobox Album See Wikipedia WikiProject Albums Name InSoc Recombinant Type Remix Artist Information Society band Information Society Cover Released April 6, 1999 Recorded Genre Synthpop Length Label Cleopatra Records Cleopatra Producer Reviews Last album Don t Be Afraid album Don t Be Afraid br 1997 This album InSoc Recombinant br 1999 Next album strange haircuts cardboard guitars and computer samples br 2001 InSoc Recombinant is a compilation album of the greatest hits by synthpop band Information Society band Information Society , in various remix remixed versions. It includes, as a bonus, a CD ROM with five music videos. Track listing What s on Your Mind Pure Energy CKB Remix Closing In Rosetta Stone band Rosetta Stone Mix Peace & Love, Inc. Biokraft Mix Think Information Society song Think Spahn Ranch band Spahn Ranch Mix What s on Your Mind? Pure Energy Girl Eats Boy Mix Walking Away Le ther Strip Mix Going Going Gone Razed in Black Mix What s on Your Mind? Pure Energy Judson Leach and the Exhibition Mix Empty Astralasia Mix Ending World Electric Hellfire Club Mix On the Outside THC band THC Mix What s on Your Mind? Pure Energy David J Remix The Brazilian release included InSoc s cover of Madonna entertainer Madonna s Express Yourself Madonna song Express Yourself previously released in the compilation Virgin Voices A Tribute To Madonna . http www.insoc.com.br mix recomb.php All the remixes of What s On Your Mind? Pure Energy were previously released on a 12 single the year before. Video listing What s on Your Mind Pure Energy Walking Away Repetition Think Peace & Love, Inc Category Information Society albums Category 1999 remix albums 1990s electronic album stub 1990s compilation album stub ... more details
Orphan date November 2006 Recombinant Human Dragon is an internet based multimedia installation exploring issues of cultural ownership and re use. It is also home to the musical artists Beastmaster Romance and The Earl of Bandwidth . Manifesto excerpts We too often confuse doing an activity with being entertained. We too readily accept passive entertainment as a substitute for active participation in culture, consumption as a substitute for production. We too often convince ourselves to be satisfied with cultural opportunities which ask us to invest nothing other than money. The project of recombinant cultural production asserts the necessity of hybrids, the impossibility of inventing anything completely new. Works of culture always borrow from, build off of, and make reference to past works, whether they like it or not. Originality is a myth that belongs with the epic works of 19th romanticism. Leave it there. You cannot create something from nothing. One needs raw materials. The process of creation requires appropriation. Influences Dadaism Situationist International Situationism External links http recombinanthumandragon.com Recombinant Human Dragon Website Category Artist groups and collectives Artist stub ... more details
File Paul Berg in 1980.jpg thumb right Paul Berg , a leading researcher in the field of recombinant DNA ... Sanger . The Asilomar Conference on Recombinant DNA was an influential academic conference conference organized by Paul Berg ref First recombinant DNA. The Human Genome Project. http www.genome.gov ... Statement of the Asilomar Conference on Recombinant DNA Molecules . Proc. Nat. Acad. Sci. Vol. 72, No. 6 ... to ensure the safety of recombinant DNA technology. The conference also placed scientific research ... recombinant Paul Berg and Maxine F. Singer. The recombinant DNA controversy Twenty years later . Proc ..., scientists worldwide had halted experiments using recombinant DNA technology, which entailed combining DNAs from different organisms. ref name asilomar ref name recombinant After the establishment of the guidelines ... 1995 , pp. 9011 12 ref Background recombinant DNA technology Recombinant DNA technology arose as a result ... that were reflected in new capacities to manipulate DNA. ref Susan Wright. Recombinant DNA Technology ... was recombinant DNA technology. Experimental design This technology entails the joining ... of the first individuals to develop recombinant DNA technology was a biochemist at Stanford by the name of Paul Berg. ref Paul Berg and Maxine F. Singer. The recombinant DNA controversy Twenty years later ... safety ramifications of this new technology. This committee, called the Committee on Recombinant DNA ... involving recombinant DNA technology. ref Carmen, Ira H. Cloning and the Constitution An Inquiry ..., 1985 ref Asilomar Conference Established principles The Asilomar Conference on Recombinant DNA ... in 1975. The main goal of the conference was to address the biohazards presented by recombinant ... , and Maxine F. Singer . Summary Statement of the Asilomar Conference on Recombinant DNA Molecules ... the use of biological barriers to limit the spread of recombinant DNA. Such biological barriers included ... information indicated that the recombinant DNA could not either alter appreciably the ecological ... more details
refimprove date June 2011 A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strain s. Families of recombinant inbred strains numbering from ... to differences in phenotype in model organisms. Recombinant inbred strains or lines ... elegans C. elegans and rat . History The origins and history of recombinant inbred strains are described ... and recombinant inbred lines journal Genetics journal Genetics year 2007 volume 176 issue 2 pages ... utility of recombinant inbred strains is mapping analysis of complex polygenic traits was obvious ... large effects quasi Mendelian loci . One of the initial motivations to use recombinant inbred strains ... of recombinant inbred mice high resolution consensus maps for complex trait analysis journal ... author Broman KW title The genomes of recombinant inbred lines journal Genetics year 2005 volume ... of using recombinant inbred strains and other genetic reference panels shifted to the ability to assemble ... and plant and animal research. Use Recombinant inbred strains are now widely used in systems ... of recombinant inbred strains under several different conditions e.g., baseline environment versus ... and environmental effects and their interactions. Genetics Chromosomes of recombinant inbred strains ... from the parental strains. In the case of a typical mouse recombinant inbred strain made by crossing maternal strain BALB cBy C with paternal strain C57BL 6By B called a CXB recombinant inbred strain ... of recombinant inbred strains, the greater the power and resolution with which phenotypes can be mapped ... mobility of proteins. Somewhat larger families of recombinant inbred strains were generated ... of recombinant inbred strains for mapping was significantly improved thanks to higher density ... extant mouse and rat recombinant inbred strains were regenotyped at many thousands of SNP markers, providing ... more details
The following is a list of notable proteins that are generated from recombinant DNA , using biomolecular engineering , focusing on those that are used in human and veterinary medicine. In many cases, recombinant human proteins have replaced the original animal derived version used in medicine. The prefix rh for human recombinant appears less and less in the literature. A much larger number of recombinant proteins is used in the research laboratory. These include both commercially available proteins for example most of the enzymes used in the molecular biology laboratory , and those that are generated in the course specific research projects. Human recombinants that largely replaced animal or harvested from human types Growth hormone Human growth hormone rHGH Humatrope from Eli Lilly and Company Lilly and Serostim from Serono replaced cadaver harvested human growth hormone Biosynthetic Insulin human insulin BHI Humulin from Eli Lilly and Company Lilly and Novolin from Novo Nordisk among others largely replaced bovine and porcine insulin for human therapy. Some prefer to continue using the animal sourced preparations, as there is some evidence that synthetic insulin varieties are more likely to induce Diabetic hypoglycemia Hypoglycemic unawareness hypoglycemia unawareness . Remaining manufacturers of highly purified animal sourced insulin include the U.K. s Wockhardt Ltd. headquartered in India , Poland s Polfa Tarchomin S.A., Argentina s Laboratorios Beta S.A., and China s Wanbang Biopharma Co. Follicle stimulating hormone FSH as a recombinant gonadotropin preparation replaced Serono s Pergonal which was previously isolated from post menopausal female urine Factor VIII Kogenate from Bayer replaced blood harvested factor VIII Human recombinants with recombination as only source Erythropoietin EPO Epogen from Amgen Granulocyte colony stimulating factor G CSF filgrastim sold as Neupogen from Amgen pegfilgrastim sold as Neulasta alpha glactosidase A Fabrazyme by Genzyme alpha ... more details
Orphan date February 2011 barelinks date June 2011 The Recombinant Immunotoxin Collaborative Group RICG is a group of scientists specialising in immunology, biochemistry and molecular biology from the United Kingdom and Italy. The group is working toward the development of genetically engineered immunotoxins made from monoclonal antibody fragments genetically fused to either saporin or Pseudomonas exotoxin PE for the treatment of human hematological malignancies such as leukaemia , lymphoma and multiple myeloma . The RICG was formed in 2005 and originally consisted of Dr Aldo Ceriotti and Dr M. Serena Fabbrini IBBA, CNR, Milan , Professor Marco Colombatti University of Verona , Professor Rodolfo Ippoliti University of L Aquila , Dr Alessandro Pini University of Siena and Drs David Flavell & Sopsamorn Flavell University of Southampton Medical School & Leukaemia Busters . Dr Alessandro Pini left the group in 2007. The RICG was successful in producing a range of recombinant immunotoxins based on the monoclonal antibodies 4KB128 and OKT10 targeting CD22 and CD38 , respectively on normal and malignant lymphoid cells. This development work was undertaken with funding from the UK based charity Leukaemia Busters ref http leukaemiabusters.org.uk ref under the direction of Dr David Flavell , the charity s Scientific Director ref http www.leukaemiabusters.org.uk pages background.aspx ref The group disbanded in 2009 due to funding difficulties following the global economic crisis but despite this the former members continue to collaborate in areas of mutual interest. In this respect some of the group members and their respective institutes have recently entered into a collaboration agreement with the Italian pharmaceutical company Domp ref http www.dompe.com website ref based in L Aquila, central Italy with the intention of developing one of the RICG produced recombinant immunotoxins for investigation in early phase clinical trials for patients with leukaemia and lymphoma. References ... more details
Enzo Paoletti and Dennis Panicali at the New York State department of Health, devised a Strategy to produce recombinant DNA vaccines by using genetic engineering techniques. They were able to transform ordinary smallpox vaccine into vaccines that may be able to prevent other diseases. What Paoletti and his colleague, Virologist Dennis Panicali, set out to do was to alter the DNA of cowpox virus by inserting a gene from another virus namely herpes, hepatitis B or influenza . These efforts resulted, amongst others in the development of a commercial Hepatitis B vaccine which is now widely used. ref References ref http www.time.com time magazine article 0,9171,921366,00.html Time Magazine article on Paoletti s work http www.thefreelibrary.com A vaccine for all seasons 3B genetic engineering is remodeling the... a03815488 Article on Paoletti s work http www.thefreelibrary.com A vaccine for all seasons 3B genetic engineering is remodeling the... a03815488 Paoletti s vaccine for herpes Category Italian inventions references Paoletti, Enzo, Bernard R. Lipinskas, Carol Samsonoff, Susan Mercer, and Dennis Panicali 1984 Construction of Live Vaccines Using Genetically Engineered Poxviruses Biological Activity of Vaccinia Virus Recombinants Expressing the Hepatitis B Virus Surface Antigen and the Herpes Simplex Virus Glycoprotein D Proc. Natl. Acad. Sci. USA 81 193 197 Panicali, Dennis, Stephen W. Davis, Randall L. Weinberg, Enzo Paoletti 1983 Construction of Live Vaccines by Using Genetically Engineered Poxviruses Biological Activity of Recombinant Vaccinia Virus Expressing Influenza Virus Hemagglutinin Proc. Natl. Acad. Sci. USA 80 5364 5368 US Patent 4722848 Method for immunizing animals with synthetically modified vaccinia virus ... more details
prose date February 2011 Recombinant adeno associated virus rAAV based genome engineering is a genome editing platform centered around the use of rAAV vectors that enables insertion, deletion or substitiution of DNA sequences into the genomes of live mammalian cells. The technique builds on Capecchi and Smithies Nobel Prize winning discovery that homologous recombination HR , a natural DNA repair mechanism, can be harnessed to perform precise genome alterations in mice. rAAV mediated genome improves the efficiency of this technique to permit genome engineering in any pre established and differentiated human cell line, Citation needed date March 2011 which in contrast to mouse ES cells, have low rates of HR. The technique has been widely adopted for use in engineering human cell lines to generate isogenic human disease models . Citation needed date March 2011 It has also been used to optimize bioproducer cell lines for the biomanufacturing of protein vaccines and therapeutics. In addition, due to the non pathogenic nature of rAAV, it has emerged as a desirable vector for performing gene therapy in live patients. Citation needed date March 2011 rAAV Vector The rAAV genome is built of single stranded deoxyribonucleic acid ssDNA , either positive or negative sensed, which is about 4.7 kilobase long. These single stranded DNA viral vectors have high transduction rates and have a unique property of stimulating endogenous HR without causing double strand DNA breaks in the genome, which is typical of other homing endonuclease mediated genome editing methods. gallery File Targeting vectors 2005 onwards.jpg Diagram of a typical rAAV vector source http horizondiscovery.com technology gene targeting gallery Capabilities Users can design a rAAV vector to any target genomic locus and perform ... cell gene knockouts using recombinant adeno associated viruses journal Nucleic Acids Res. volume ...?view long&pmid 20026587 refend Reflist Category Biochemistry Category Recombinant proteins ... more details
Orphan date February 2011 Heterologous expression is to gene Expression express a gene or part of a gene in a host organism , which does not naturally have this gene or gene fragment.This is mainly done in recombinant DNA technology . See also Recombinant DNA Category Biotechnology Biotech stub ... more details
Unreferenced stub date December 2009 Orphan date December 2009 Alvircept sudotox is a form of recombinant CD4 derived from Pneumonas aeruginosa exotoxin A, or PE40. DEFAULTSORT Alvircept Sudotox Category Glycoproteins Category Clusters of differentiation Biochem stub ... more details
RBST or rBST may refer to Randomized binary search tree , a computer data structure Rare Breeds Survival Trust , a UK charity Recombinant bovine somatotropin usually rBST , a synthetic growth hormone controversially used in dairy farming disambig ... more details
lowercase rDNA rDNA may stand for ribosomal DNA recombinant DNA RDNA is also an acronym for Reformed Druids of North America disambig Long comment to avoid being listed on short pages de RDNA ... more details
RTPA may refer to Radiotelevisi n del Principado de Asturias , a public broadcasting company in Asturias, Spain. rtPA may refer to Tissue plasminogen activator Recombinant tissue plasminogen activator , a protein involved in the breakdown of blood clots. disambig ... more details
Moroctocog alfa is a glycoprotein trade name ReFacto and an recombinant Antihemophilic Factor genetically engineered from Chinese hamster ovary CHO cell line ref http www.abopharmaceuticals.com ProductSheets Refacto.pdf ref . It is manufactured by Genetics Institute, Inc. and used to control and prevent hemorrhagic bleeding and prophylaxis associated with surgery or to reduce the number of spontaneous bleeding episodes in patients with hemophilia A. It is partially a recombinant coagulation factor VIII since it has an amino acid sequence which compares to the 90 80 kDa form of factor VIII BDDrFVIII ref http www.ema.europa.eu humandocs PDFs EPAR Refacto 058999en6.pdf ref . It also has posttranslational modifications which are similar to those of the plasma derived molecule. It can not prevent hemorrhagic bleeding associated with von Willebrand s disease since it s not a von Willebrand factor . References reflist Category Recombinant proteins blood drug stub ... more details
In Molecular biology , an insert is a piece of DNA that is inserted into a larger DNA Cloning vector vector by a recombinant DNA technique, such as DNA ligase ligation or Genetic recombination recombination . This allows it to be multiplied, selected, further manipulated or Gene expression expressed in a Host biology host organism . Category Molecular biology fr Insert biologie mol culaire ... more details
RIL may refer to Radio Interface Layer , a software interface used in a mobile device to communicate via mobile networks RDF Inference Language , a means of expressing expert systems rules and queries that operate on RDF models Reliance Industries Limited, a corporation in India Rice Lane railway station , England National Rail station code RIL Recombinant inbred strain Recombinant Inbred Lines , a population derived from multiply inbred strains in order to study complex genetic traits The FAA and IATA identifier for Garfield County Regional Airport in Rifle, Colorado See also Rill disambiguation de Ril eo RIL it RIL no RIL ... more details
CD4 immunoadhesin is a recombinant fusion protein consisting of a combination of CD4 and the fragment crystallizable region . External links MeshName CD4 Immunoadhesins cite journal author Fletcher CV title Nonlinear pharmacokinetics of high dose recombinant fusion protein CD4 IgG2 PRO 542 observed in HIV 1 infected children journal J. Allergy Clin. Immunol. volume 119 issue 3 pages 747 50 year 2007 pmid 17336619 doi 10.1016 j.jaci.2006.10.045 pmc 1910693 author2 DeVille JG author3 Samson PM display authors 3 last4 Moyejr first4 J last5 Church first5 J last6 Spiegel first6 H last7 Palumbo first7 P last8 Fenton first8 T last9 Smith first9 M Category Engineered proteins protein stub ... more details
Human influenza hemagglutinin HA is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98 106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. ref http www.millipore.com catalogue item 05 904 ref Propriety The actual HA tag is as follows 5 TAC CCA TAC GAT GTT CCA GAT TAC GCT 3 . The amino acid sequence is YPYDVPDYA References reflist Category Influenza Category Viral structural proteins ... more details
phenotype s ABC 244 parental genotype, shows no recombination ABc 81 recombinant between B and C AbC 23 double recombinant Abc 152 recombinant between A and B aBC 148 recombinant between A and B aBc 27 double recombinant abC 89 recombinant between B and C abc 236 parental genotype, shows ... more details
58 ref Recombinant Factor XIII Recombinant Factor XIII rFXIII is the only prospective drug alternative ... in Spring 2010. ref name Recombinant Factor XIII Although it is a recombinant protein, rFXIII ... bleeding episodes. ref cite web author Clinical Trials at Novo Nordisk. title Evaluation of Recombinant ... more details
2011 They are now made using recombinant DNA techniques, are produced in bacteria like E. coli and are called recombinant immunotoxins . Function The antibody or other targeting moiety binds to an antigen ...? These patients were treated with the recombinant immunotoxin, BL22 , which targets the CD22 cell ... recombinant immunotoxin BL22 in chemotherapy resistant hairy cell leukemia journal N. Engl. J. Med ... more details
Maltose Binding Protein MBP is a part of the maltose maltodextrin system of Escherichia coli , which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many protein s and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons. Use MBP is used to increase the solubility of recombinant protein s expressed in E. coli . In these systems, the protein of interest is often expressed as a MBP fusion protein , preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest X is often cleaved from MBP with a specific protease . Protein X can then be separated from MBP by ion exchange chromatography. See also Fluorescent glucose biosensors Glutathione S transferase External links http www.bio.mtu.edu campbell purifica.htm N Terminal Fusion of Target Protein to Maltose Binding Protein at Michigan Technological University MeshName maltose binding protein http www.nature.com nprot journal v2 n2 full nprot.2007.50.html Generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6 maltose binding protein fusion tag Category Proteins protein stub ... more details
Encyclopedia
top
