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Encyclopedia results for T7 DNA polymerase

T7 DNA polymerase





Encyclopedia results for T7 DNA polymerase

  1. T7 DNA polymerase

    The T7 DNA polymerase of the T7 phage T7 bacteriophage is a DNA dependent DNA polymerase responsible for the fast rate of T7 phage DNA replication in vivo . The polymerase consists of a 1 1 complex of the viral T7 gene 5 protein 80kDA and the E. coli thioredoxin 12kDA . It lacks a 5 3 exonuclease domain, but the 3 5 exonuclease activities are approximately 1000 fold greater than that of Klenow fragment . ref Cite journal author Sambrook, J., Russell, D.W. title Molecular Cloning A Laboratory Manual, Third edition journal Cold Spring Harbor Laboratory Press location Cold Spring Harbor, New York, 2001. year 2001 ref The exonuclease activity appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis ref Cite journal author LECHNER, R. L. AND RICHARDSON, C. C. journal J. Biol. Chem. title A preformed, topologically stable replication fork. volume 258 year 1983 pages 11185 11196 pmid 6885816 issue 18 ref This polymerase is unique due to its considerable processivity , or ability to stay on DNA for a greater than average number of base pairs. It is also suitable for site directed mutagenesis ref Cite journal author BEBENEK, K. et al. journal Nucl. Acids Res. title The use of native T7 DNA polymerase for site directed mutagenesis. volume 17 year 1989 pages 5408 doi 10.1093 nar 17.13.5408 pmid 2668888 issue 13 pmc 318147 ref but is not recommended for DNA sequencing applications. ref Cite journal author Doublie S., Tabor S., Long A., Richardson C., and Ellenberger T. journal Nature title Crystal Structure of a Bacteriophage T7 DNA Replication complex at 2.2 A Resolution. volume 391 year 1998 pages 251 258 doi 10.1038 34593 pmid 9440688 issue 6664 ref References references Refbegin 2 PBB Further reading citations Refend The PBB Controls template provides controls for Protein Box Bot, please see Template PBB Controls for details. PBB ... citations yes Category DNA replication Enzyme stub ...   more details



  1. DNA polymerase

    members from this family include the extensively studied T7 DNA polymerase , as well as the eukaryotic mitochondrial DNA Polymerase . Among the repair polymerases are Escherichia coli DNA ...infobox enzyme Name DNA directed DNA polymerase EC number 2.7.7.7 CAS number 9012 90 2 IUBMB EC number ... helix turn helix motifs in human DNA polymerase beta based on pdb file http www.rcsb.org pdb explore.do?structureId 7ICG 7ICG A DNA polymerase is an enzyme the suffix ase is used to identify enzymes ... are best known for their Negative feedback feedback role in DNA replication , in which the polymerase ... Image DNA polymerase.svg thumb 200px right DNA polymerase with proofreading ability DNA polymerase ... strand in a 5 3 direction. No known DNA polymerase is able to begin a new chain de novo . DNA ... pair is recognized, DNA polymerase reverses its direction by one base pair of DNA. The 3 5 exonuclease ... B virus DNA polymerase . These may selectively replicate viral DNA through a variety of mechanisms. Retroviruses encode an unusual DNA polymerase called reverse transcriptase , which is an RNA dependent DNA polymerase RdDp . It polymerizes DNA from a template of RNA . DNA polymerase families Infobox protein family Symbol DNA pol A Name DNA polymerase family A image PDB 2hht EBI.jpg width caption ... family Symbol DNA pol B Name DNA polymerase family B image PDB 2dy4 EBI.jpg width caption crystal ... Infobox protein family Symbol DNA pol B 2 Name DNA polymerase type B, organellar and viral image PDB 1xhz EBI.jpg width caption phi29 dna polymerase, orthorhombic crystal form, ssdna complex Pfam PF03175 ... chromosomal replicative enzymes. DNA Polymerase III alpha subunit from E. coli is the catalytic .... Xeroderma pigmentosum variant XPV patients for instance have mutations in the gene encoding DNA polymerase ... Wittschieben B Wood RD date 2008 title DNA polymerase zeta pol zeta in higher eukaryotes journal Cell ... RT Some viruses also encode special DNA polymerases, such as Hepatitis B virus DNA polymerase. These may ...   more details



  1. T7 RNA polymerase

    T7 polymerase binds to DNA and transcribes it. Related proteins Related family members include phage T3 and SP6 RNA polymerase s, but this family is also related to the mitochondrial RNA polymerase. The T7 ..., T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two different phage promoters e.g., T7 and T3, or T7 and SP6 in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different polymerases. The enzyme ...Image T7 RNA polymerase.jpg thumb right T7 RNA Polymerase blue producing mRNA light blue from a double stranded DNA template orange . T7 RNA Polymerase is an RNA polymerase from the T7 phage T7 bacteriophage that catalyzes the formation of RNA in the 5 3 direction. Activity T7 polymerase is extremely Promoter biology promoter specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a DNA template and Mg sup 2 sup ion as cofactor for the synthesis of RNA. It has a very low error rate. T7 polymerase has a molecular weight of 99 kDa. Structure T7 polymerase has been crystallised in several forms and the structures placed in the Protein Data Bank PDB . The different ... RNA polymerases, T7 polymerase is not inhibited by the antibiotic rifampicin . Nevertheless, many common ..., Theis K, Gong P title Structure and function in promoter escape by T7 RNA polymerase journal Prog ... 6603 05 80008 X url Cite journal author Sousa R, Mukherjee S title T7 RNA polymerase journal Prog ... T7 RNA polymerase or, the virtues of simplicity journal Cell. Mol. Biol. Res. volume 39 issue 4 pages ... of T7 RNA polymerase and the heterogeneity of transcription elongation complexes journal J. Biol ... T7 RNA Polymerase Enzymology http openwetware.org wiki In vitro transcription with T7 RNA polymerase OpenWetWare polymerases Category RNA Molecular cell biology stub Enzyme stub de T7 RNA Polymerase it T7 RNA polimerasi tr T7 RNA polimeraz zh T7RNA ...   more details



  1. T7 DNA helicase

    T7 DNA helicase is a hexameric motor protein that uses energy from Thymidine triphosphate dTTP hydrolysis to process unidirectionally along single stranded DNA , separating the two strands as it progresses. Crystal structure The crystal structure was solved to 3.0 resolution in 2000, ref cite journal doi 10.1016 S0092 8674 00 80871 5 author Singleton MR, Sawaya MR, Ellenberger T, Wigley DB title Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides journal Cell volume 101 issue 6 pages 589 600 year 2000 month June pmid 10892646 url http linkinghub.elsevier.com retrieve pii S0092 8674 00 80871 5 ref as shown in the figure. In A , notice that the separate subunits appear to be anchored through interactions between an alpha helix and an adjacent subunit. In B , there are six sets of three loops. The red loop, known as loop II, contains three lysine residues and is thought to be involved in binding the ssDNA that is fed through the center of the enzyme. Mechanism of sequential dTTP hydrolysis Crampton et al. have proposed a mechanism for the ssDNA dependent hydrolysis of dTTP by T7 DNA helicase as shown in the figure below. ref cite journal author Crampton DJ, Mukherjee S, Richardson CC title DNA induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase journal Mol. Cell volume 21 issue 2 pages 165 74 year 2006 month January pmid 16427007 doi 10.1016 j.molcel.2005.11.027 url http linkinghub.elsevier.com retrieve pii S1097 2765 05 01815 0 ref In their model, protein loops located on each ... MM, Patel SS, Egelman EH title DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase journal Nat. Struct. Biol. volume 3 issue 9 pages 740 3 year 1996 month September pmid 8784344 ref See also Helicase References reflist External links MeshName T7 DNA Primase Helicase Protein DNA replication Category Molecular biology Category Enzymes Category DNA replication ...   more details



  1. Prokaryotic DNA polymerase

    There are 5 known Prokaryotic DNA polymerases DNA polymerase I Pol I implicated in DNA repair has 5 3 polymerase activity, and both 3 5 exonuclease activity proofreading and 5 3 exonuclease activity RNA primer removal . DNA polymerase II Pol II involved in repairing damaged DNA has 3 5 exonuclease activity. DNA polymerase III holoenzyme Pol III the main polymerase in bacteria responsible for elongation has 3 5 exonuclease activity proofreading . DNA polymerase IV Pol IV a Y family DNA polymerase, Involved in SOS repair and translesion repair. DNA polymerase V Pol V a Y family DNA polymerase participates in bypassing DNA damage, involved in SOS repair and translesion repair. See also DNA polymerase Polymerases DNA replication Category EC 2.7.7 Category DNA replication Category DNA ...   more details



  1. DNA polymerase eta

    DNA polymerase eta Pol is a eukaryotic DNA polymerase involved in the DNA repair Translesion synthesis DNA repair by translesion synthesis . The gene encoding DNA polymerase eta is POLH , also known as XPV, because loss of this gene results in the disease Xeroderma Pigmentosum Variant. Polymerase eta is particularly important for allowing accurate translesion synthesis of DNA damage resulting from ultraviolet radiation or UV. References refbegin http www.ncbi.nlm.nih.gov sites entrez?db gene&cmd retrieve&list uids 5429 refend Category DNA replication ...   more details



  1. DNA polymerase II

    Unreferenced stub auto yes date December 2009 This article is about the DNA Polymerase. For the RNA Polymerase, see RNA polymerase II DNA polymerase II also known as DNA Pol II or Pol II is a prokaryote prokaryotic DNA polymerase most likely involved in DNA repair . The enzyme is 90 kDa in size and is coded by the POLB polB gene . DNA Pol II can synthesize DNA new base pairs at an average rate of between 40 and 50 nucleotides second. Strains lacking the gene show no defect in growth or replication. Synthesis of Pol II is induced during the stationary phase of cell growth. This is a phase in which little growth and DNA synthesis occurs. It is also a phase in which the DNA can accumulate mutation damage such as short gaps, which act as a block to DNA Pol III . Under these circumstances, Pol II helps to overcome the problem because it can reinitiate DNA synthesis downstream of gaps. Pol II has a low error rate but it is much too slow to be of any use in normal DNA synthesis. Pol II differs from Pol I in that it lacks a 5 3 exonuclease activity, and cannot use a nicked duplex template. See also DNA replication . DEFAULTSORT Dna Polymerase Ii Category DNA replication Category EC 2.7.7 Category Enzymes Transferase stub cs DNA polymer za II it DNA polimerasi II ...   more details



  1. DNA polymerase delta

    DNA polymerase delta is an enzyme complex found in eukaryote s that is involved in DNA replication and DNA repair repair , and it consists of the proliferating cell nuclear antigen PCNA , the multisubunit replication factor C , and the 4 subunit polymerase complex POLD1 , POLD2 , POLD3 , and POLD4 . ref name pmid16934752 cite journal author Liu G, Warbrick E title The p66 and p12 subunits of DNA polymerase delta are modified by ubiquitin and ubiquitin like proteins journal Biochem. Biophys. Res. Commun. volume 349 issue 1 pages 360 6 year 2006 month October pmid 16934752 doi 10.1016 j.bbrc.2006.08.049 url issn ref References Reflist External links MeshName DNA polymerase delta DNA replication enzyme stub NLM content Category EC 2.7.7 cs DNA polymer za ...   more details



  1. DNA polymerase I

    molecular biology 5 3 forward DNA Dependent DNA polymerase activity, requiring a 3 Primer molecular ... DNA polymerase I as a reverse transcriptase author Ricchetti M, Buc H year 1993 month February journal Embo Journal volume 12 issue 2 pages 387 396 pmc 413221 ref In the replication process, DNA Polymerase ... that Polymerase I was not the enzyme responsible for most DNA synthesis &mdash DNA replication ... Strain with a Mutation affecting DNA Polymerase author Paula de Lucia coauthors John Cairns ref Cairns ... them for DNA polymerase activity. The 3,478th clone contained the polA mutant, which was named ... The eureka enzyme the discovery of DNA polymerase author Errol C. Friedberg ref It was not until the discovery of DNA polymerase III that the main replicative DNA polymerase was finally identified. Research applications DNA polymerase I obtained from E. coli is used extensively for molecular biology ... molecule called the Klenow fragment , widely used in molecular biology . Exposure of DNA polymerase ... the DNA polymerase and proofreading activities. See also DNA polymerase II DNA polymerase III References Reflist Polymerases DNA replication DEFAULTSORT Dna Polymerase I Category EC 2.7.7 Category DNA ...Image PolymeraseDomains.jpg right thumb 300px center Functional domains in the Klenow Fragment left and DNA Polymerase I right . center DNA Polymerase I or Pol I is an enzyme that participates in the process of DNA replication and is exclusively found in prokaryote s. It is composed of 928 amino acids ... journal J. Biol. Chem. volume 233 issue 1 pages 163 170 pmid 13563462 ref it was the first known DNA polymerase and, indeed, the first known of any kind of polymerase . It was initially characterized ... biology proofreading A 5 3 forward exonuclease activity mediating nick translation during DNA repair . A Directionality molecular biology 5 3 forward RNA Dependent DNA polymerase activity. Pol I operates on RNA templates with considerably lower efficiency 0.1&ndash 0.4 than it does DNA templates ...   more details



  1. DNA polymerase III holoenzyme

    Pol III can also refer to HNoMS Pol III , a Norwegian guard vessel from WWII DNA polymerase III holoenzyme ... Name Bacterial DNA polymerase III alpha subunit image width caption crystal structure of the catalytic alpha subunit of e. coli replicative dna polymerase iii Pfam PF07733 Pfam clan InterPro IPR011708 ... pol3 delta Name DNA polymerase III, delta subunit image PDB 1xxh EBI.jpg width caption atpgs bound ... family OPM protein CAZy CDD Infobox protein family Symbol DNA pol3 tau 4 Name DNA polymerase III subunits ... 5 Name DNA polymerase III tau subunit V interacting with alpha image width caption solution structure of the c terminal 14 kda domain of the tau subunit from escherichia coli dna polymerase iii Pfam ... Infobox protein family Symbol DNA pol3 theta Name DNA polymerase III, theta subunit image PDB 2ido ... protein family Symbol DNA pol3 chi Name DNA polymerase III chi subunit, HolC image PDB 1em8 EBI.jpg ... the polymerase bound to the DNA. 2 units which acts to dimerize two of the core enzymes , , and ... DNA polymerase III synthesizes base pairs at a rate of around 1000 nucleotides per second. ref ... stranded DNA, is synthesized by primase an RNA polymerase for RNA , for DNA , for polymerase RNA ribose ... progresses and the replisome moves forward, DNA polymerase III arrives at the RNA primer ... G U A U Polymerase Pol RNA primer III hydrogen bonding C A T A G C A T C C DNA template ss DNA single stranded DNA deoxyribose sugar phosphate backbone Synthesis of DNA DNA polymerase III will then synthesize ... or lagging strand Okazaki fragment of the DNA. DNA polymerase III has a high processivity and therefore ... onto the DNA strands. DNA deoxyribose sugar phosphate backbone G U A U C G T A G G Polymerase Pol ... is removed by DNA polymerase I via the process of nick translation . The removal of the RNA primer ... of DNA replication. See also DNA replication DNA polymerase Beta clamp http www.pdbe.org quips?story BetaClamp Clamping down on pathogenic bacteria how to shut down a key DNA polymerase complex ...   more details



  1. Pfu DNA polymerase

    For the measure of viral plaques Plaque forming unit DISPLAYTITLE Pfu DNA polymerase Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus , where it functions in vivo to replicate the organism s DNA . In vitro , Pfu is used to amplify DNA in polymerase chain reaction PCR processes, where the enzyme serves the central function of copying a new strand of DNA during each extension step. Proofreading ability of Pfu polymerase The main difference between Pfu and alternative enzymes is Pfu s superior thermostability and proofreading properties compared to Taq DNA polymerase Taq DNA polymerase . Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3 to 5 exonuclease proofreading activity, meaning that it works its way along the DNA from the 5 end to the 3 end and corrects nucleotide misincorporation errors. This means that Pfu DNA polymerase generated PCR fragments will have fewer errors than Taq generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq . Citation needed date May 2012 Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6 mutated products when amplifying 1 kb fragments using PCR. However, Pfu is slower and typically requires 1&ndash 2 minutes per cycle to amplify 1kb of DNA at 72 C. Using Pfu DNA polymerase in PCR reactions also results in blunt ended PCR products. Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase ... their work as Lundberg et al., A High Fidelity Thermostable DNA Polymerase Isolated from Pyrococcus ... 3Den Patent 5,545,552 Category Microbiology Category DNA replication Category EC 2.7.7 de Pfu Polymerase id Pfu DNA polimerase ...   more details



  1. Eukaryotic DNA polymerase

    strand . POLB DNA polymerase beta Pol Implicated in repairing DNA, in base excision repair and gap filling synthesis. POLG , POLG2 Pol Replicates and repairs mitochondrial DNA and has proofreading 3 5 exonuclease activity. POLD1 , POLD2 , POLD3 , POLD4 DNA polymerase delta Pol Highly processive and has proofreading 3 5 exonuclease activity. Thought to be the main polymerase involved in lagging ... Scott D McCulloch Thomas A Kunkel 148 161 . POLH , POLI , POLK , DNA polymerase eta , , POLK , and Rev1 are Y family DNA polymerases and Pol is a B family DNA polymerase. These polymerases are involved in the bypass of DNA damage. ref cite journal author I. Prakash, S. Johnson, R. E. Prakash, L. date 2005 title Eukaryotic translesion synthesis DNA polymerases specificity of structure and function ... that deal with the elongation , and have proofreading ability 3 5 exonuclease . See also DNA polymerase References reflist Polymerases DNA replication Category EC 2.7.7 Category DNA replication ...There are at least 15 Eukaryotic DNA polymerases ref cite journal author I. Hubscher, U. Maga, G. Spadari, S. date 2002 title Eukaryotic DNA polymerases journal Annual Review of Biochemistry volume 71 page 133 63 pmid 12045093 doi 10.1146 annurev.biochem.71.090501.150041 pages 133 ref POLA1 , POLA2 Polymerase DNA directed , alpha 1 Pol forms a complex with a small catalytic PriS and a large noncatalytic PriL subunit ref cite journal author Elizabeth R. Barry Stephen D. Bell date 12 2006 title DNA ... synthesizing an RNA primer , and then with DNA Pol elongating that primer with DNA nucleotides ... of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases journal Cell ... to pol , and thought to be the main polymerase involved in leading strand synthesis ref cite journal author Pursell, Z.F. et al. date 2007 title Yeast DNA Polymerase Participates in Leading Strand DNA Replication journal Science volume 317 page 127 130 pmid 17615360 doi 10.1126 science.1144067 ...   more details



  1. DNA polymerase lambda

    PBB geneid 27343 DNA polymerase lambda , also known as POLL , is a protein that, in humans, is encoded by the POLLA gene . ref name entrez cite web title Entrez Gene POLL polymerase DNA directed , lambda ... of the DNA polymerase Family X X family of DNA polymerase s. It is thought to resynthesize missing nucleotides during non homologous end joining , a pathway of DNA repair Double strand breaks DNA ... A, Wilson TE title DNA joint dependence of pol X family polymerase action in nonhomologous end joining ... al. title Implication of DNA polymerase lambda in alignment based gap filling for nonhomologous DNA ... shows that, unlike the DNA polymerases that catalyze DNA replication , pol makes extensive contacts with the 5 phosphate of the downstream DNA strand. This allows the polymerase to stabilize the two ... L, Kunkel TA, Pedersen LC title A structural solution for the DNA polymerase lambda dependent repair of DNA gaps with minimal homology journal Mol. Cell volume 13 issue 4 pages 561 72 year 2004 month ... participate in base excision repair , where it provides backup activity in the absence of DNA polymerase ... EK, Prasad R, Shock DD, Hou EW, Beard WA, Wilson SH title DNA polymerase lambda mediates a back ... Identification of an intrinsic 5 deoxyribose 5 phosphate lyase activity in human DNA polymerase ... Pol is structurally and functionally related to DNA polymerase mu pol , another member of the X ... kappa light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu ... cite journal author Maga G, Villani G, Ramadan K, et al. title Human DNA polymerase lambda ... B, Storck S, Delbos F, Cocea L, Gupta N, Weill JC, Reynaud CA title Two novel human and mouse DNA polymerases ... Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells journal DNA Repair Amst. volume 6 issue 6 pages 869 75 year 2007 month June pmid 17363341 ... url ref Besides its catalytic polymerase domain, pol has an 8 kDa domain and a BRCT domain . The 8 ...   more details



  1. ?29 DNA polymerase

    29 DNA polymerase is an enzyme from the 29 phage bacteriophage 29 . It is being increasingly used ... 29 DNA polymerase. Symmetrical mode of DNA replication journal J. Biol. Chem. volume 264 issue 15 ... mutagenesis of the YCDTDS amino acid motif of the phi 29 DNA polymerase journal Gene volume 94 ... phi 29 DNA polymerase, a proofreading enzyme journal J. Biol. Chem. volume 267 issue 4 pages ... DNA polymerase and a novel primer at elevated temperature journal BMC Res Notes volume 2 issue pages ... labeling of DNA via rolling circle amplification RCA polymerase 29. journal Eur J Med Chem volume ... DNA polymerase amplification performance by fusion of DNA binding motifs. journal Proc Natl Acad ... M title phi29 DNA polymerase active site role of residue Val250 as metal dNTP complex ligand and in protein ... JM, Salas M, de Vega M title Functional importance of bacteriophage phi29 DNA polymerase residue Tyr148 ... of viral DNA genomes using phi29 polymerase. journal Trends Microbiol volume 17 issue 5 ... with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature ... DNA polymerase RNA detection and analysis in vitro and in situ by target RNA primed RCA. journal ... of the TPR2 subdomain movement in the activities of phi29 DNA polymerase. journal Nucleic Acids ... BJ, Dunn JJ title Rapid detection and identification of a pathogen s DNA using Phi29 DNA polymerase ... E, Reagin M, Vidali A title Improved multiple displacement amplification with phi29 DNA polymerase ..., L zaro JM, de Vega M title The bacteriophage phi29 DNA polymerase. journal IUBMB Life volume 60 issue ... Silander K, Saarela J title Whole genome amplification with Phi29 DNA polymerase to enable genetic ... of polynucleotide substrates for Phi29 DNA polymerase 3 5 RNase activity of the enzyme. journal ... Vega M title Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring ... M, Steitz TA title Structures of phi29 DNA polymerase complexed with substrate the mechanism of translocation ...   more details



  1. DNA polymerase mu

    PBB geneid 27434 DNA polymerase mu is a human protein encoded by the POLM gene . ref name entrez cite web title Entrez Gene POLM polymerase DNA directed , mu url http www.ncbi.nlm.nih.gov sites entrez?Db gene&Cmd ShowDetailView&TermToSearch 27434 accessdate ref Function Pol is a member of the DNA polymerase Family X X family of DNA polymerase DNA polymerases . It participates in resynthesis of damaged or missing nucleotides during the non homologous end joining non homologous end joining NHEJ pathway of DNA repair . ref name pmid15964833 cite journal author Daley JM, Laan RL, Suresh A, Wilson TE title DNA joint dependence of pol X family polymerase action in nonhomologous end joining journal J. Biol. Chem. volume 280 issue 32 pages 29030 7 year 2005 month August pmid 15964833 doi 10.1074 jbc.M505277200 url ref Pol interacts with Ku protein Ku and LIG4 DNA ligase IV , which also participate in NHEJ. ref name pmid12077346 cite journal author Mahajan KN, Nick McElhinny SA, Mitchell BS, Ramsden DA title Association of DNA polymerase mu pol mu with Ku and ligase IV role for pol mu in end joining double strand break repair journal Mol. Cell. Biol. volume 22 issue 14 pages 5194 202 year 2002 month July pmid 12077346 pmc 139779 doi 10.1128 MCB.22.14.5194 5202.2002 url ref It is structurally and functionally related to DNA polymerase lambda pol , and, like pol , pol has a BRCT domain that is thought to mediate interactions with other DNA repair proteins. ref name pmid15242403 cite journal author Nick McElhinny SA, Ramsden DA title Sibling rivalry competition between Pol X family members in V D J recombination and general double strand break repair journal Immunol. Rev. volume ... deoxynucleotidyl transferase TdT , a specialized DNA polymerase that adds random nucleotides to DNA ... is impaired in mice deficient for DNA polymerase mu journal Immunity volume 19 issue 2 pages ... Bertocci B, De Smet A, Weill JC, Reynaud CA title Nonoverlapping functions of DNA polymerases ...   more details



  1. DNA polymerase beta

    edit Polymerase DNA directed , beta , also known as POLB , is an enzyme that, in humans, is encoded by the POLB gene . ref name entrez cite web title Entrez Gene POLB polymerase DNA directed , beta ... ref Function In Eukaryote eukaryotic cells, DNA polymerase beta POLB performs base excision repair ... , and drug resistance. ref name entrez Regulation of expression DNA polymerase beta maintains genome ... of DNA polymerase beta in cell results in a mutator phenotype and a decreased sensitivity ... Deregulated DNA polymerase beta induces chromosome instability and tumorigenesis journal Cancer Res ... SH title DNA polymerase beta expression differences in selected human tumors and cell lines journal .... ref name pmid12674496 cite journal author He F, Yang XP, Srivastava DK, Wilson SH title DNA polymerase ... author Narayan S, He F, Wilson SH title Activation of the human DNA polymerase beta promoter by a DNA ... structure within the 3 UTR of DNA polymerase beta mRNA acts as a post transcriptional regulatory element ... to be determined. Interactions DNA polymerase beta has been shown to Protein protein interaction ... with DNA polymerase beta and has a potential role in cellular phenotype journal Cancer Res. volume ... DNA polymerase beta and the XRCC1 protein journal EMBO J. volume 15 issue 23 pages 6662 70 publisher ... of a DNA polymerase beta variant journal Biochemistry volume 40 issue 30 pages 9005 13 publisher ... laysummary laysource laydate quote doi 10.1021 bi0028789 ref See also Polymerase DNA directed , alpha ... journal author Date T title Two regions in human DNA polymerase beta mRNA suppress translation in Escherichia ... U, Ghosh L, Banerjee S title DNA polymerase beta mutations in human colorectal cancer journal Cancer ... of DNA polymerase beta by in vitro phosphorylation with protein kinase C journal J. Biol ... SenGupta DN title Sequence of human DNA polymerase beta mRNA obtained through cDNA cloning journal ... A, Collins J, Wilson SH title Characterization of DNA polymerase beta mRNA cell cycle and growth ...   more details



  1. T7

    T7 or T 7 may refer to In vehicles T7 Combat Car , a prototype vehicle for the US Army T 7 rocket , a sounding rocket Fuji T 7 , a Japanese primary trainer aircraft Trikke 7, a 3 wheeled cambering vehicle with 7 wheels A model of the OS T1000 train of the Oslo Metro Beechcraft Model 18 T 7 Navigator , a trainer aircraft used by the United States armed forces between 1937 and 1970 In biology T7 phage , a virus used in the study of biological systems, from which came T7 DNA Helicase , a hexameric motor protein T7 RNA polymerase , an RNA polymerase that catalyzes the formation of RNA In anatomy 7th Thoracic vertebrae thoracic vertebra Thoracic spinal nerve 7 Other uses 2C T 7 , a hallucinogenic phenethylamine A Terminator series Terminator model Sony Cyber shot DSC T7, a digital camera manufactured by Sony Twin Jet IATA airline code Tango Seven, company in charge to registrer aircraft in San Marino T Seven, aka Judith Hildebrandt, a singer and former member of Mr. President band The abbreviation of Triple Seven An abbreviation of the Terrorist 7 countries Letter NumberCombDisambig de T7 es T7 fr T7 it T7 ja T 7 sv T7 ...   more details



  1. Hepatitis B virus DNA polymerase

    Image HBV genome.png right thumb 300px The genome organisation of HBV. The genes overlap. ORF P, in blue, encodes Hepatitis B virus DNA polymerase Hepatitis B virus DNA polymerase is a hepatitis B viral protein . ref name pmid10051501 cite journal author Ono Nita SK, Kato N, Shiratori Y, et al. title YMDD motif in hepatitis B virus DNA polymerase influences on replication and lamivudine resistance A study by in vitro full length viral DNA transfection journal Hepatology volume 29 issue 3 pages 939 45 year 1999 month March pmid 10051501 doi 10.1002 hep.510290340 ref ref name pmid8903366 cite journal author Shaw T, Mok SS, Locarnini SA title Inhibition of hepatitis B virus DNA polymerase by enantiomers of penciclovir triphosphate and metabolic basis for selective inhibition of HBV replication by penciclovir journal Hepatology volume 24 issue 5 pages 996 1002 year 1996 month November pmid 8903366 doi 10.1002 hep.510240504 ref It encodes a DNA polymerase . References reflist Viral proteins virus stub Category Viral proteins es Virus de la hepatitis B ADN polimerasa ...   more details



  1. Polymerase (DNA directed), alpha 1

    PBB geneid 5422 DNA polymerase alpha catalytic subunit is an enzyme that in humans is encoded by the POLA1 gene . ref name entrez cite web title Entrez Gene POLA1 polymerase DNA directed , alpha 1 url ... synthesis of Okazaki fragments. Interactions DNA dependent polymerase alpha Pol has been shown to Protein ... S year 1997 month Nov. title Phosphorylated retinoblastoma protein stimulates DNA polymerase alpha ... Apr. title Functional association of poly ADP ribose polymerase with DNA polymerase alpha primase ... of replication in the c myc gene, interacts with a catalytic subunit of DNA polymerase alpha and stimulates ... 9 year 2003 pmid 12546699 doi 10.1042 BST0310266 cite journal author Fisher PA, Korn D title DNA polymerase ... journal author Dornreiter I, Erdile LF, Gilbert IU, et al. title Interaction of DNA polymerase alpha ... protein A on the initiation of DNA synthesis by DNA polymerase alpha primase. journal Mol. Cell ... Martelli AM, Cocco L, Manzoli FA title On the association of DNA polymerase alpha activity with the nuclear ... Human DNA polymerase alpha gene sequences controlling expression in cycling and serum stimulated ... author Hsi KL, Copeland WC, Wang TS title Human DNA polymerase alpha catalytic polypeptide binds ConA ... author Wang TS, Pearson BE, Suomalainen HA, et al. title Assignment of the gene for human DNA polymerase ... requires DNA polymerase delta. journal J. Biol. Chem. volume 263 issue 1 pages 501 10 year 1988 pmid 3335506 doi cite journal author Wong SW, Wahl AF, Yuan PM, et al. title Human DNA polymerase alpha ... of human DNA polymerase alpha by alpha 1 antichymotrypsin. journal Cancer Res. volume 46 issue ... populations of DNA polymerase alpha in HeLa cells. journal J. Mol. Biol. volume 192 issue 1 pages ... C, Ulrich RG title Inhibition of DNA replication and DNA polymerase alpha activity by monoclonal anti DNA polymerase alpha immunoglobulin G and F ab fragments. journal Biochemistry volume 24 issue ... S, Hu SZ, et al. title Intracellular localization of human DNA polymerase alpha with monoclonal ...   more details



  1. Polymerase

    Image Taq polimerase.png thumb Structure of Taq polymerase Taq DNA polymerase A polymerase EC 2.7.7.6 7 19 48 49 is an enzyme whose central function is associated with polymer s of nucleic acid s such as RNA and DNA . The primary function of a polymerase is the polymerization of new DNA or RNA against an existing DNA or RNA template in the processes of DNA replication replication and Transcription genetics transcription . In association with a cluster of other enzymes and proteins, they take nucleotide s from solvent, and catalyse the synthesis of a polynucleotide sequence against a nucleotide template strand using base pairing interactions. It is an accident of history that the enzymes responsible for the catalytic production of other biopolymer s are not also referred to as polymerases. One particular polymerase, from the thermophile thermophilic bacteria bacterium , Thermus aquaticus Taq Protein data bank PDB http www.rcsb.org pdb cgi explore.cgi?pid 288631034363198&pdbId 1BGX 1BGX , EC 2.7.7.7 is of vital commercial importance due to its use in the polymerase chain reaction , a widely used technique of molecular biology . Other well known polymerases include Terminal Deoxynucleotidyl Transferase TDT , which lends diversity to antibody heavy chains Reverse Transcriptase , an enzyme used by RNA retrovirus es like HIV , which is used to create a complementary strand to the preexisting strand of viral RNA before it can be integrated into the DNA of the host biology host cell biology cell . It is also a major target for antiviral drugs. See also DNA polymerase DNA polymerase I DNA polymerase II DNA polymerase III holoenzyme DNA Polymerase IV DinB SOS response SOS repair polymerase RNA polymerase RNA polymerase I RNA polymerase II RNA polymerase III T7 RNA polymerase Phosphotransferases DNA replication Category EC 2.7.7 Category Enzymes ca Polimerasa da Polymerase de Polymerase es Polimerasa fr Polym rase it Polimerasi pl Polimerazy pt Polimerase tr Polimeraz uk ...   more details



  1. T7 phage

    phage s DNA polymerase . T7 polymerase uses E. coli s endogenous thioredoxin as a sliding clamp ... functions to hold the polymerase onto the DNA, which increases the rate of synthesis initiation, the process by which a polymerase binds to DNA, is time consuming. The T7 promoter sequence is used ... T7 DNA and the locations of T7 genetic elements. J. Mol. Biol. 166 4 , 477 535 url http www.sciencedirect.com ... in translocating bacteriophage T7 DNA into the infected cell 2010 Virology, 398 2 , pp. 176 186.http ... . When co transfected with T7 RNA polymerase T7 RNAP into cultured human osteosarcoma 143B cells ...Taxobox name Enterobacteria phage T7 virus group I ordo Caudovirales familia Podoviridae genus T7 like viruses species T7 phage File T7 Phage.jpg Bacteriophage T7 is a bacteriophage capable of infecting ... O157 H7 E. coli O157 H7 , a strain of E. coli which can cause foodborne illness . Bacteriophage T7 is a DNA virus that has a lytic life cycle. The T7 bacteriophage has several properties that make it an ideal phage for experimentation. Discovery Bacteriophage T7 was identified in 1945 as a member ... of T7 was likely studied by d Herelle in the 1920s ref name d Herelle1926 d Herelle, F. 1926 . The Bacteriophage and Its Behavior. Baltimore, MD Williams & Wilkins ref . Hosts T7 grows on rough strains ... strains ref name Molineux2006 Molineux, I. J. 2006 . Chapter 20 The T7 group. In The Bacteriophages .... Genome The genome of phage T7 ref cite web title Genome of bacteriophage T7 url http www.ncbi.nlm.nih.gov ... Uniprot reference proteome of bacteriophage T7 url http www.uniprot.org uniprot ?query organism 10760 keyword 1185 ref . Life cycle T7 has a short life cycle of 17 min at 37 C, i.e. the time from infection ..., high fitness strains of T7 have been isolated with a latent period of only 11 min at 37 C growing under ... ref . Infection of Host Bacteria The T7 phage recognizes receptors on the surface of E.coli cells. It adheres to the cell surface by the tail fibers. In some strains of T7 the tail fibers are replaced ...   more details



  1. RNA polymerase

    in viruses Image RNA pol.jpg thumb T7 RNA polymerase producing a mRNA green from a DNA template. The protein ... of the above techniques. See also Transcription genetics DNA polymerase T7 RNA polymerase RNA polymerase ...infobox enzyme Name DNA Directed RNA Polymerase EC number 2.7.7.6 CAS number 9014 24 8 IUBMB EC number 2 7 7 6 GO code 0034062 image width 260px caption Check the EC number again RNA polymerase RNAP or RNApol , also known as DNA dependent RNA polymerase , is an enzyme that produces RNA . In cell biology cell s, RNAP is necessary for constructing RNA chains using DNA gene s as templates, a process called Transcription genetics transcription . RNA polymerase enzymes are essential to life and are found ... . In contrast to DNA polymerase , RNAP includes helicase activity, therefore no separate enzyme is needed to unwind DNA. RNA polymerase action RNA polymerase binding in bacteria involves the the sigma ... for genes required at high temperatures heat shock genes . After binding to the DNA, the RNA polymerase ... via RNA polymerase s catalytic activity and recommence DNA scrunching to achieve promoter escape ... activity that characterizes the proofreading activity found in DNA polymerase, there is evidence ... to form RNA polymerase holoenzyme. Sigma reduces the affinity of RNAP for nonspecific DNA while ... AJ, Jensen MB, Dalmay T, Baulcombe DC title RNA polymerase IV directs silencing of endogenous DNA ... is bacteriophage T7 RNA polymerase . Other viruses use a RNA dependent RNA polymerase RNA dependent ... http www.dnai.org DNAi DNA Interactive, including information and Flash clips on RNA Polymerase. MeshName ... dna template RNA Polymerase Synthesis RNA from DNA Template Polymerases Category Gene expression ... author Jerard Hurwitz year 2005 month December title The Discovery of RNA Polymerase journal Journal ... polymerase during various stages of the transcription process. ref http nobelprize.org nobel prizes ... Transcription label en.jpg thumb An electron micrograph of DNA DNA strands decorated by hundreds ...   more details



  1. DNA

    ref File T7 RNA polymerase.jpg thumb left T7 RNA polymerase blue producing a mRNA green from a DNA ... the mechanism of polymerase induced strand separation of double helical DNA journal Mol Microbiol ... and topoisomerase . Next, one DNA polymerase produces the Replication fork leading strand copy. Another DNA polymerase binds to the Replication fork lagging strand . This enzyme makes discontinuous ... by an enzyme called DNA polymerase . This enzyme makes the complementary strand by finding the correct ... June 2, 2012 small yes pp move indef File DNA Structure Key Labelled.pn NoBB.png thumb right 340px The structure of the DNA double helix . The atoms in the structure are colour coded by Chemical element ... thumb The structure of part of a DNA double helix Deoxyribonucleic acid IPAc en audio en us Deoxyribonucleic acid.ogg d i k s i r a b . n ju k l e . k s d DNA is a nucleic ... known living organism s with the exception of RNA virus es . The DNA segments carrying this genetic information are called gene s. Likewise, other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information. Along with RNA and proteins , DNA is one of the three major macromolecules that are essential for all known forms of life . DNA consists of two long ... is read by copying stretches of DNA into the related nucleic acid RNA in a process called transcription genetics transcription . Within cells DNA is organized into long structures called chromosome s. During cell division these chromosomes are duplicated in the process of DNA replication , providing ... s, Fungus fungi , and protist s store most of their DNA inside the cell nucleus and some of their DNA ..., prokaryote s bacteria and archaea store their DNA only in the cytoplasm . Within the chromosomes, chromatin proteins such as histone s compact and organize DNA. These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed ...   more details



  1. Polymerase stuttering

    Polymerase stuttering is the process by which a polymerase Transcription genetics transcribe s a nucleotide several times without progressing further on the mRNA chain. It is often used in addition of poly A tail s or 5 cap capping mRNA chains by less complex organism s such as viruses. Process A polymerase may undergo stuttering as a probability controlled event, hence it is not explicitly controlled by any mechanisms in the translation process. Generally, it is a result of many short repeated ribosomal frameshift frameshift s on a slippery sequence of nucleotide s on the mRNA strand ref Anderson EC, Hunt SL, Jackson RJ. Internal initiation of translation from the human rhinovirus 2 internal ribosome entry site requires the binding of Unr to two distinct sites on the 5 untranslated region. J Gen Virol. 2007 Nov 88 Pt 11 3043 52. ref . However, the frameshift is restricted to one in some cases two ref Mauro VP, Chappell SA, Dresios J. Analysis of polymerase shunting during translation initiation in eukaryotic mRNAs. Methods Enzymol. 2007 429 323 54. ref nucleotides with a pseudoknot or choke point s on both sides of the sequence. Examples A polymerase that exhibits this behavior is RNA dependent RNA polymerase , present in many RNA viruses . Reverse transcriptase has also been observed to undergo this polymerase stuttering ref Kurzynska Kokorniak A, Jamburuthugoda VK, Bibillo A, Eickbush TH. DNA directed DNA polymerase and strand displacement activity of the reverse transcriptase encoded by the R2 retrotransposon. J Mol Biol. 2007 Nov 23 374 2 322 33. Epub 2007 Sep 20. ref . Literature reflist Category Genetics ...   more details



  1. Taq polymerase

    DISPLAYTITLE Taq polymerase Infobox protein family Symbol Taq exonuc Name Taq polymerase, exonuclease image taq.png width caption DNA polymerase bound to a DNA octamer Pfam PF09281 Pfam clan InterPro IPR015361 SMART PROSITE MEROPS SCOP 1qtm TCDB OPM family OPM protein CAZy CDD Taq polymerase IPAc en icon t k p l m r e z is a thermostable DNA polymerase named after the thermophilic bacterium ... in polymerase chain reaction PCR , a method for greatly amplifying short segments of DNA . T. aquaticus is a bacterium that lives in hot springs and hydrothermal vent s, and Taq polymerase was identified ... of DNA with a thermostable DNA polymerase. journal Science journal Science year 1988 volume 239 pages 487 91 doi 10.1126 science.2448875 pmid 2448875 issue 4839 ref Therefore it replaced the DNA polymerase ... characterization of full length Thermus aquaticus DNA polymerase ... journal PCR Methods Appl ... KR and Kunkel TA year 1988 title Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase journal ... DNA polymerases have been isolated from other thermophilic bacteria and archaea, such as Pfu DNA polymerase Pfu DNA polymerase , possessing a proofreading activity, and are being used instead ... product, thus enabling DNA ligase ligation of the PCR product into the plasmid vector. Taq polymerase ... hybridize to each strand of a target DNA, and adding DNA polymerase to the reaction. This led to exponential DNA replication , ref cite journal author Mullis KB title The unusual origin of the polymerase ... the DNA polymerase that was in use before the discovery of Taq polymerase, the Klenow fragment of the DNA ... 12158 1 Curran, Chris, Bio Medicine, Dec. 7, 1999 ref See also DNA polymerase I DNA replication ... et al. cite journal author Chien A, Edgar DB, Trela JM year 1976 title Deoxyribonucleic acid polymerase ... replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 C. ref name Lawyer et al ... motion in Taq polymerase revealed by neutron spin echo spectroscopy journal Proc. Natl. Acad. Sci ...   more details




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