The year 1564 in science and technology included many events, some of which are listed here.

Births

• February 15 - Galileo Galilei, Pisan astronomer (died 1642).
• March 9 - David Fabricius, Frisian astronomer (died 1617).
• approx. date - Pierre Richer de Belleval, French botanist (died 1632).

Deaths

• April Pierre Belon, French naturalist (born 1517) (murdered)
• October 15 Vesalius, Flemish anatomist (born 1514)
• October 18 Johannes Acronius Frisius, German physician and mathematician (born 1520)
• Charles Estienne, French anatomist (born 1504)

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