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 The A-DNA structure.
Structure A-DNA is fairly similar to B-DNA given that it is a right-handed double helix with major and minor grooves. However, as shown in the comparison table below, there is a slight increase in the number of base pairs per rotation (resulting in a tighter rotation angle), and smaller rise/turn. This results in a deepening of the major groove and a shallowing of the minor. Predicting A-DNA structure An algorithm for predicting the propensity of a sequence to flip from B-DNA to A-DNA was developed by Beth Basham, Gary Schroth, and P. Shing Ho at Oregon State University.[1] The abstract of their work describes this algorithm: The ability to predict macromolecular conformations from sequence and thermodynamic principles has long been coveted but generally has not been achieved. We show that differences in the hydration of DNA surfaces can be used to distinguish between sequences that form A- and B-DNA. From this, a "triplet code" of A-DNA propensities was derived as energetic rules for predicting A-DNA formation. This code correctly predicted > 90% of A- and B-DNA sequences in crystals and correlates with A-DNA formation in solution. Thus, with our previous studies on Z-DNA, we now have a single method to predict the relative stability of sequences in the three standard DNA duplex conformations.[1] Comparison Geometries of the Most Common DNA Forms  Side view of A-, B-, and Z-DNA.  The helix axis of A-, B-, and Z-DNA. | Geometry attribute: | A-form | B-form | Z-form | | Helix sense | right-handed | right-handed | left-handed | | Repeating unit | 1 bp | 1 bp | 2 bp | | Rotation/bp | 33.6 | 35.9 | 60 /2 | | Mean bp/turn | 11 | 10.5 | 12 | | Inclination of bp to axis | +19 | 1.2 | 9 | | Rise/bp along axis | 2.4 (0.24 nm) | 3.4 (0.34 nm) | 3.7 (0.37 nm) | | Rise/turn of helix | 24.6 (2.46 nm) | 33.2 (3.32 nm) | 45.6 (4.56 nm) | | Mean propeller twist | +18 | +16 | 0 | | Glycosyl angle | anti | anti | pyrimidine: anti,
purine: syn | | Sugar pucker | C3'-endo | C2'-endo | C: C2'-endo,
G: C2'-exo | | Diameter | 23 (2.3 nm) | 20 (2.0 nm) | 18 (1.8 nm) | References - ↑ a b
See also External links cs:A-DNA es:ADN-A pt:DNA-A ru:A- sr:A-DNK tr:A-DNA uk:A- zh:A-DNA
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