AOT may refer to:

• Airports of Thailand, an airport operator
• Ahead of Time, see AOT compiler
• Aosta Airport, Italy (IATA code)
• A.O. Trikala, a football club in Trikala, Greece
• Army of Two, a video game
• Aerosol OT, a common chemical reagent, see Dioctyl sodium sulfonsuccinate
• "Arse over Tit" a slang expression meaning "head over heels"
• Autorit organisatrice de transports, a French legal structure for the provision of public transport

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