Ace in the Hole may refer to:

Music

• Ace in the Hole (band), the backup band for George Strait
• "Ace in the Hole," a 1909 jazz standard written by George D. Mitchell & James E. Dempsey
• "Ace in the Hole" (Cole Porter song)
• "Ace in the Hole" (George Strait song) written by Dennis Adkins
• "Ace in the Hole," a song by Paul Simon on the album One-Trick Pony
• "Ace in the Hole," a song by Dave Van Ronk on the album In the Tradition

Other uses

• Ace in the Hole (film)
• Ace in the Hole (cartoon)
• Ace in the Hole (anthology), edited by George R. R. Martin
• Ace in the Hole, a 2009 sitcom pilot starring Adam Carolla
• Having an ace as a hole card in poker